Tg(Prnp)23Olds
Transgene Detail
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| Symbol: |
Tg(Prnp)23Olds |
| Name: |
transgene insertion 23, Michael Oldstone |
| MGI ID: |
MGI:6766457 |
| Synonyms: |
Tg23 |
| Transgene: |
Tg(Prnp)23Olds Location: unknown
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| Alliance: |
Tg(Prnp)23Olds page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Prnp)23Olds expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Prnp (MGI:97769) |
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Mutation details: Mice with this transgene express a prion protein under control of the regulatory sequences of the mouse prion protein gene (Prnp) originally contained in the "half-genomic" vector pHGPrp, which includes 6 kb of 5'- and 2.2 kb of 3'-flanking DNA, exon 1, intron 1, and exon 2 joined to exon 3 of the mouse Prnp gene. C-terminal sequences encoding the GPI signal sequence were removed from a serine codon at residue 233 to an SfoI site approximately 400 bases downstream, and these were replaced by a stop codon and useful restriction sites. The expressed protein is a mouse prion protein terminating at Ser230, just before the C terminal membrane anchor of the PRNP precursor protein in the mature protein. Two lines were generated; Tg23 and Tg44.
(J:140306)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:140306 Chesebro B, et al., Anchorless prion protein results in infectious amyloid disease without clinical scrapie. Science. 2005 Jun 3;308(5727):1435-9 |
| All: |
5 reference(s) |
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Analysis Tools
