Gene Expression Data
Assay Details
Assay
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Reference:
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J:309394
Kwon HJ, et al., Hippo pathway/Yap regulates primary enamel knot and dental cusp patterning in tooth morphogenesis. Cell Tissue Res. 2015 Nov;362(2):447-51
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6764471
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Gene symbol:
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Yap1
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Gene name:
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yes-associated protein 1
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Results
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Specimen
1g:
embryonic day 13.5
(more )
Note: Double labeled: green - Yap1; red - TOPRO-3.
Specimen
1g:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Yap1; red - TOPRO-3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth bud |
Present |
Regionally restricted |
1g
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At bud stage, expression in basal cell layer of dental epithelium.
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TS21: tooth epithelium |
Present |
Regionally restricted |
1g
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At bud stage, expression in basal cell layer of dental epithelium.
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Specimen
1h:
embryonic day 14.5
(more )
Note: Double labeled: green - Yap1; red - TOPRO-3.
Specimen
1h:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
|
Double labeled: green - Yap1; red - TOPRO-3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cap stage tooth |
Present |
Regionally restricted |
1h
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At cap stage, nuclear expression (co-expression with TOPRO-3) in pattern similar to that of Ki67.
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Specimen
1h':
embryonic day 14.5
(more )
Note: Double labeled: green - Yap1; red - TOPRO-3.
Specimen
1h':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Yap1; red - TOPRO-3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: primary enamel knot |
Weak |
Regionally restricted |
1h'
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In primary enamel knot (PEK), expression reduced in cytoplasm and completely absent from nucleus.
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Specimen
1i:
embryonic day 16.5
(more )
Note: Double labeled: green - Yap1; red - TOPRO-3.
Specimen
1i:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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Double labeled: green - Yap1; red - TOPRO-3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: bell stage tooth |
Present |
Regionally restricted |
1i
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At bell stage, authors report expression profile similar to those in primary enamel knot at cap stage.
|
Specimen
1i':
embryonic day 16.5
(more )
Note: Double labeled: green - Yap1; red - TOPRO-3.
Specimen
1i':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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Double labeled: green - Yap1; red - TOPRO-3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: secondary enamel knot |
Weak |
Regionally restricted |
1i'
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In secondary enamel knot (SEK), no nuclear expression.
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