Tg(Mchr1-cre/ERT2)2Nbe
Transgene Detail
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| Symbol: |
Tg(Mchr1-cre/ERT2)2Nbe |
| Name: |
transgene insertion 2, Nicolas F Berbari |
| MGI ID: |
MGI:6763060 |
| Synonyms: |
Mchr1-CreER |
| Transgene: |
Tg(Mchr1-cre/ERT2)2Nbe Location: unknown
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| Alliance: |
Tg(Mchr1-cre/ERT2)2Nbe page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Mchr1-cre/ERT2)2Nbe expression driven by
1 gene
Transgene expression driven by:
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Mutation details: A cre/ERT2 expression sequence was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting Mchr1-cre/ERT2 BAC transgene was purified for subsequent male pronuclear injection to establish transgenic founder animals. Two lines 2 and 3 were generated with similar expression patterns.
(J:266416)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Mchr1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:266416 Engle SE, et al., A CreER mouse to study melanin concentrating hormone signaling in the developing brain. Genesis. 2018 Aug;56(8):e23217 |
| All: |
1 reference(s) |
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Analysis Tools
