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Gene Expression Data
Assay Details
Assay
Reference: J:115783 Kiernan AE, et al., The Notch ligand JAG1 is required for sensory progenitor development in the mammalian inner ear. PLoS Genet. 2006 Jan;2(1):e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6763027
Gene symbol: Myo7a
Gene name: myosin VIIA
Antibody: Anti-Myo7a
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 6A: embryonic day 18.5 (more )
Note: Apex region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6A Expressed in inner and outer hair cells.
Specimen 6B: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Apex region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6B Expressed in inner hair cells. Outer hair cells were not present in the mutant.
Specimen 6C: embryonic day 18.5 (more )
Note: Middle region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6C Expressed in inner and outer hair cells.
Specimen 6D: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Middle region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6D Expressed in both inner and occasionally outer hair cells, although their patterning was clearly abnormal.
Specimen 6E: embryonic day 18.5 (more )
Note: Base region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6E Expressed in inner and outer hair cells.
Specimen 6F: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Base region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6F No hair cells were present in the base region.
Specimen 6G: embryonic day 18.5 (more )
Note: Utricle region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: utricle epithelium Present Regionally restricted 6G Expressed in the hair cells.
Specimen 6H: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Utricle region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: utricle epithelium Present Regionally restricted 6H The utricular macula is extremely small with very few positive hair cells.
Specimen 6I: embryonic day 18.5 (more )
Note: Saccule region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: saccule epithelium Present Regionally restricted 6I Expressed in the hair cells.
Specimen 6J: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Saccule region. Double labeled: red - Myo7a; green - S100a1 (assay MGI:6763028).
Structure Level Pattern Image Note
TS26: saccule epithelium Present Regionally restricted 6J Expressed in the hair cells. Hair cell differentiation appeared relatively unaffected, although the entire saccular structure was shaped differently than in the controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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