Hoxb9 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173614 Paul D, et al., HOX genes are expressed in bovine and mouse oocytes and early embryos. Mol Reprod Dev. 2011 Jun;78(6):436-49
Assay type:	RT-PCR
MGI Accession ID:	MGI:6761873
Gene symbol:	Hoxb9
Gene name:	homeobox B9

Probe:	Hoxb9-pI, Hoxb9-pJ
Assay notes:	Assay was nested RT-PCR; MGI:6761867 was used as the outer primer pair. Results are expressed as the percentage of positive pools.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
o	P adult (a)	TS28: oocyte	Present (c)	Not Specified; total RNA	involves: C57BL/6J * DBA/2		Female
1 c	E0.5 (a)	TS1: 1-cell stage embryo	Present (d)	Not Specified; total RNA	involves: C57BL/6J * DBA/2		Not Specified
2 c	E1.0 (a)	TS2: 2-cell stage embryo	Present (d)	Not Specified; total RNA	involves: C57BL/6J * DBA/2		Not Specified
M	E3.0 (a)	TS3: compacted morula	Strong (e)	Not Specified; total RNA	involves: C57BL/6J * DBA/2		Not Specified
B	E3.5 (a)	TS4: blastocyst	Strong (e)	Not Specified; total RNA	involves: C57BL/6J * DBA/2		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression detected with a high frequency at all stages.
(c) 75% expression in oocytes.
(d) 67% expression in 1-cell and 2-cell embryos.
(e) 100% expression in morulae and blastocysts.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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