Antibody:	Gprc5b antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2A	Strong expression in maternal vascular endothelial cells (MECs), glycogen cells (GICs) and stromal cells (SCs).
TS21: labyrinthine zone	Strong	Regionally restricted	2A	Strong expression in labyrinthine vascular endothelial cells (LECs) derived from the fetus. No expression in trophoblast cells of labyrinthine layer.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2B	Strong expression in maternal vascular endothelial cells (MECs), glycogen cells (GICs) and stromal cells (SCs).No remarkable change in intensity of expression in GICs and SCs during gestation period.
TS23: labyrinthine zone	Strong	Regionally restricted	2B	Strong expression in labyrinthine vascular endothelial cells (LECs) derived from the fetus. No expression in trophoblast cells of labyrinthine layer.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2C	Strong expression in glycogen cells (GICs) and stromal cells (SCs). No remarkable change in intensity of expression in GICs and SCs during gestation period. No expression in maternal vascular endothelial cells (MECs).
TS25: labyrinthine zone	Absent		2C	No expression in labyrinthine vascular endothelial cells (LECs). No expression in trophoblast cells of labyrinthine layer.

Structure	Level	Pattern	Image	Note
TS21: labyrinthine zone	Strong	Regionally restricted	2D	Strong expression in labyrinthine vascular endothelial cells LECs) derived from the fetus.

Structure	Level	Pattern	Image	Note
TS23: labyrinthine zone	Strong	Regionally restricted	2E	Strong expression in labyrinthine vascular endothelial cells (LECs) derived from the fetus. No expression in trophoblast cells of labyrinthine layer.

Structure	Level	Pattern	Image	Note
TS25: labyrinthine zone	Absent		2F	No expression in labyrinthine vascular endothelial cells (LECs). No expression in trophoblast cells of labyrinthine layer.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2M	Strong expression in maternal vascular endothelial cells (MECs), glycogen cells (GICs) and stromal cells (SCs).No remarkable change in intensity of expression in GICs and SCs during gestation period.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2O	Strong expression in glycogen cells (GICs) and stromal cells (SCs). No remarkable change in intensity of expression in GICs and SCs during gestation period. No expression in maternal vascular endothelial cells (MECs).

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2Q	Strong expression in glycogen cells (GICs) and stromal cells (SCs). No remarkable change in intensity of expression in GICs and SCs during gestation period. No expression in maternal vascular endothelial cells (MECs).

Structure	Level	Pattern	Image	Note
TS21: yolk sac blood vessel	Strong	Regionally restricted	2S	Strong expression in vascular endothelial cells and visceral mesoderm.
TS21: yolk sac mesenchyme	Strong	Regionally restricted	2S	Strong expression in vascular endothelial cells and visceral mesoderm.

Structure	Level	Pattern	Image	Note
TS23: yolk sac blood vessel	Strong	Regionally restricted	2U	Strong expression in vascular endothelial cells and visceral mesoderm. No change in intensity of expression during gestation.
TS23: yolk sac mesenchyme	Strong	Regionally restricted	2U	Strong expression in vascular endothelial cells and visceral mesoderm. No change in intensity of expression during gestation.

Structure	Level	Pattern	Image	Note
TS25: yolk sac blood vessel	Strong	Regionally restricted	2W	Strong expression in vascular endothelial cells and visceral mesoderm. No change in intensity of expression during gestation.
TS25: yolk sac mesenchyme	Strong	Regionally restricted	2W	Strong expression in vascular endothelial cells and visceral mesoderm. No change in intensity of expression during gestation.