TgTn(pb-Gt(ROSA)26Sor/CAG-EYFP*,-tdTomato*,-KDR*,-mEYFP*,-Kdr*,-mKate2*)#Ben
Transgene Detail
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| Symbol: |
TgTn(pb-Gt(ROSA)26Sor/CAG-EYFP*,-tdTomato*,-KDR*,-mEYFP*,-Kdr*,-mKate2*)#Ben |
| Name: |
transgenic transposon concatemer, Rui Benedito |
| MGI ID: |
MGI:6761550 |
| Synonyms: |
iMb-Vegfr2-Mosaic, TgBAC(Rosa)26Sor(iMb-Vegfr2-Mosaic) |
| Transgene: |
TgTn(pb-Gt(ROSA)26Sor/CAG-EYFP*,-tdTomato*,-KDR*,-mEYFP*,-Kdr*,-mKate2*)#Ben Location: unknown
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| Alliance: |
TgTn(pb-Gt(ROSA)26Sor/CAG-EYFP*,-tdTomato*,-KDR*,-mEYFP*,-Kdr*,-mKate2*)#Ben page
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| Transgene Type: |
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Transgenic (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter, Transposon concatemer) |
| Mutation: |
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Transposon insertion
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TgTn(pb-Gt(ROSA)26Sor/CAG-EYFP*,-tdTomato*,-KDR*,-mEYFP*,-Kdr*,-mKate2*)#Ben expresses
2 genes
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| mouse |
Kdr (MGI:96683) |
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sequence coding for mutated mouse peptide with p.Y1173F substitution is downstream in-frame with T2A sequence which is downstream in-frame with mEYFP gene |
| human |
KDR (3791) |
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sequence coding for human peptide where extra-cellular domain was deleted is downstream in-frame with T2A sequence which is downstream in-frame with tdTomato gene |
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Mutation details: This allele has three membrane-targeted fluorescent protein sequences - each uniquely flanked with pairs of incompatible lox sites (in the same direction) inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus on BAC RP23-401D9. PiggyBAC transposon inverted repeats were added to the BAC to facilitate single-copy and complete insertion into the genome with the PiggyBAC system. The targeting vector is designed with (from 5' to 3') two copies of chicken beta-globin HS4 insulator element (2xINS; increases downstream gene expression and minimizes regulatory interference), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, the non-cytotoxic/non-fluorescent Phialidium yellow fluorescent protein variant PhiYFP with nuclear localization signal (N-PhiMut), bovine GH polyA signal sequence, a second loxN site, the MbTomato gene (non-oligomerizing tandem-dimer DsRed fluorescent protein variant tdTomato with N-terminal membrane-targeting tag), in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping) followed by in-frame mutated human KDR cDNA for the active form (where sequence coding for the extra-cellular domain was deleted), bovine GH polyA signal sequence, a second lox2272 site, the MbYFP gene (monomeric enhanced yellow fluorescent protein [mYFPA206K] with N-terminal membrane-targeting tag), in-frame T2A sequence followed by in-frame mutated mouse Kdr cDNA (with a p.Y1173F mutation that renders the residue non-phosphorylatable and abolishes the peptide's downstream signalling upon binding Vegf), bovine GH polyA signal sequence, a second loxP site, the MbKate2 gene (monomeric far-red fluorescent protein mKate2 with N-terminal membrane-targeting tag), bovine GH polyA signal sequence, and a second 2xINS sequence.
(J:252386)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:252386 Pontes-Quero S, et al., Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Cell. 2017 Aug 10;170(4):800-814.e18 |
| All: |
2 reference(s) |
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Analysis Tools
