Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Gad2tm1.1(icre/ERT2)Bcgen
Name:	glutamic acid decarboxylase 2; targeted mutation 1.1, Biocytogen LLC
MGI ID:	MGI:6761379
Synonyms:	GAD65-iCreERT2
Gene:	Gad2  Location: Chr2:22512262-22583889 bp, + strand  Genetic Position: Chr2, 15.15 cM, cytoband A-B
Alliance:	Gad2tm1.1(icre/ERT2)Bcgen page

Germline Transmission:	Earliest citation of germline transmission: J:243876
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	C57BL/6

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Gad2tm1.1(icre/ERT2)Bcgen expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Gad2 (MGI:95634)   Mutation details: A 2A-icre/ERT2 is introduced between the coding sequence of Gad2 Exon 16 and 3' UTR. Neo cassette flanked by frt sites was inserted within intron 15 to avoid disrupting poly(A) signal of Gad2 and promoter region of Apbb1ip, a gene nearby. After homologous recombination in ES cells, the neo selection cassette was removed by Flp recombinase. (J:243876)

Activity:	Tissue activity of this recombinase allele
Driver:	Gad2 (mouse) Summary of all recombinase alleles driven by Gad2. Alliance mouse Gad2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gad2 Mutation:	60 strains or lines available

Original:	J:243876 Zhong H, et al., Endocannabinoid signaling in hypothalamic circuits regulates arousal from general anesthesia in mice. J Clin Invest. 2017 Jun 01;127(6):2295-2309
All:	2 reference(s)