Tg(Gt(ROSA)26Sor-DAAM1*))#Jwng
Transgene Detail
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| Symbol: |
Tg(Gt(ROSA)26Sor-DAAM1*))#Jwng |
| Name: |
transgene insertion, Jianbo Wang |
| MGI ID: |
MGI:6761276 |
| Synonyms: |
Rosa26-deltaDAD |
| Transgene: |
Tg(Gt(ROSA)26Sor-DAAM1*))#Jwng Location: unknown
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| Alliance: |
Tg(Gt(ROSA)26Sor-DAAM1*))#Jwng page
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| Strain of Origin: |
Not Applicable
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| Transgene Type: |
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Transgenic (Conditional ready, Constitutively active) |
| Mutation: |
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Insertion
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Mutation details: The transgene consists of 206 kb BAC clone RP24-85L15 that contains the mouse Rosa26 genomic region with inserted DAD, which encodes a constitutively active form of human DAAM1 mutant into the intron 2 of Rosa26. A floxed cassette consisted of three tandem polyadenylation signals was placed in front of DAD so that DAD transcription can only be induced only upon Cre-mediated excision of the 3x poly(A) cassette. A CAG promoter was placed upstream of the floxed 3x poly(A) cassette to enhance transcription, and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was added downstream of DAD to increase mRNA stability.
(J:284141)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:284141 Li D, et al., Planar cell polarity signaling regulates polarized second heart field morphogenesis to promote both arterial and venous pole septation. Development. 2019 Oct 9;146(20):dev181719 |
| All: |
1 reference(s) |
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