Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Adgra3tm1.1(HBEGF,-cre/ERT2)Pac
Name:	adhesion G protein-coupled receptor A3; targeted mutation 1.1, Pamela Cowin
MGI ID:	MGI:6759474
Synonyms:	Adgra3cre
Gene:	Adgra3  Location: Chr5:50117293-50216338 bp, - strand  Genetic Position: Chr5, 26.85 cM
Alliance:	Adgra3tm1.1(HBEGF,-cre/ERT2)Pac page

Germline Transmission:	Earliest citation of germline transmission: J:309699
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NTac)F1

Allele Type:    Targeted (Inducible, Inserted expressed sequence, Null/knockout, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Adgra3tm1.1(HBEGF,-cre/ERT2)Pac expresses 1 gene Knock-in expresses: Organism Expressed Gene Homolog in Mouse Note human HBEGF (1839) Hbegf  (MGI:96070)   Adgra3tm1.1(HBEGF,-cre/ERT2)Pac expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Adgra3 (MGI:1917943)   Mutation details: The targeting vector contains a human diphtheria toxin receptor gene (HBEGF) fused to an enhanced green fluorescent protein (EGFP), a self-cleaving peptide T2A, an FRT-flanked neomycin cassette and cre/ERT2 inserted immediately after the first methionine codon. The construct places the diphtheria toxin receptor, EGFP, and cre/ERT2 under the control of the Adgra3 promoter, and excises 502 bp encompassing 221 bp of exon 1 and part of the following intron to generate a knockout. Mice treated with diptheria toxin die due to expression of HBEGF in vital organs. EGFP is not detected in living cells by immunofluorescence or FACS. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:309699)

Activity:	Tissue activity of this recombinase allele
Driver:	Adgra3 (mouse) Summary of all recombinase alleles driven by Adgra3. Alliance mouse Adgra3 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Adgra3 Mutation:	71 strains or lines available

Original:	J:309699 Spina E, et al., Gpr125 identifies myoepithelial progenitors at tips of lacrimal ducts and is essential for tear film. bioRxiv. 2020;
All:	2 reference(s)