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Gene Expression Data
Assay Details
Assay
Reference: J:263255 Kusumakshi S, et al., A Binary Genetic Approach to Characterize TRPM5 Cells in Mice. Chem Senses. 2015 Jul;40(6):413-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6757237
Gene symbol: Trpm5
Gene name: transient receptor potential cation channel, subfamily M, member 5
Antibody: anti-Trpm5
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 4A: postnatal week 8-20; Trpm5tm1Csz/Trpm5tm1Csz (more )
Structure Level Pattern Image Note
TS28: circumvallate papillae Absent 4A
Specimen 4C: postnatal week 8-20 (more )
Note: wild type
Structure Level Pattern Image Note
TS28: circumvallate papillae Present 4C
Specimen 4D: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: circumvallate papillae Present 4D
Specimen 4E: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: foliate papillae Present 4E
Specimen 4F: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: fungiform papillae Present 4F
Specimen 6A/B: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: limiting ridge of stomach Present Regionally restricted 6B merge , 6B TRPM5 , 6A TRPM5 , 6A merge Expression is seen in a subset of cells in the gastric groove.
Specimen 6C: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: duodenum epithelium Present Single cells 6C TRPM5 , 6C merge Expression is seen in epithelium lining the villi and glands; they may be tufted cells.
Specimen 6D: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: colon epithelium Present Single cells 6D TRPM5 , 6D merge Expression is seen in epithelium lining the villi and glands; they may be tufted cells.
Specimen 7A: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: trachea epithelium Present Single cells 7A TRPM5 , 7A tauGFP/TRPM5 Expression is seen in the lining.
Specimen 7B: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: bronchus epithelium Present Single cells 7B TRPM5 , 7B tauGFP/TRPM5 Expression is seen in the lining.
Specimen 7C: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: urethra Present 7C TRPM5 , 7C tauGFP/TRPM5
Specimen 8A/B: postnatal week 8-20; Trpm5tm1.1(cre)Uboe/Trpm5+, Gt(ROSA)26Sortm1(CAG-Mapt/GFP)Uboe/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trpm5; green - Cre reporter.
Structure Level Pattern Image Note
TS28: olfactory epithelium Present Single cells 8B TRPM5 , 8B merge , 8A TRPM5 , 8A merge Expression is seen in a population of short cells localized in the apical portion of the olfactory epithelium; they are likely microvillar cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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