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Gene Expression Data
Assay Details
Assay
Reference: J:191065 Kang M, et al., FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse. Development. 2013 Jan;140(2):267-79
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6756434
Gene symbol: Sox17
Gene name: SRY (sex determining region Y)-box 17
Antibody: anti-SOX17
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3A Fgf4+/+ E3.5: embryonic day 3.5 (more )
Note: 60 cells. Double labeled: red - Sox17; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A Fgf4+/+ E3.5 Merge , 3A Fgf4+/+ E3.5 SOX17
Specimen 3A Fgf4-/- E3.5: embryonic day 3.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 58 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Sox17; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A Fgf4-/- E3.5 Merge , 3A Fgf4-/- E3.5 SOX17 Expression is in significantly fewer cells in this mutant than in wild-type embryos.
Specimen 3A Fgf4+/+ E4.5: embryonic day 4.5 (more )
Note: 92 cells. Double labeled: red - Sox17; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A Fgf4+/+ E4.5 Merge , 3A Fgf4+/+ E4.5 SOX17
Specimen 3A Fgf4-/- E4.5: embryonic day 4.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 72 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Sox17; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A Fgf4-/- E4.5 Merge , 3A Fgf4-/- E4.5 SOX17 Expression is in significantly fewer cells in this mutant than in wild-type embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory