Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Cd209btm1.1(cre,HBEGF)Bcgen
Name:	CD209b antigen; targeted mutation 1.1, Biocytogen LLC
MGI ID:	MGI:6755214
Synonyms:	Cd209btm1.1(cre,DTR)Bcgen, SIGN-R1-Cre/DTR
Gene:	Cd209b  Location: Chr8:3967655-3976841 bp, - strand  Genetic Position: Chr8, 1.92 cM, cytoband A1.2-A1.3
Alliance:	Cd209btm1.1(cre,HBEGF)Bcgen page

Germline Transmission:	Earliest citation of germline transmission: J:288614
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	C57BL/6

Allele Type:    Targeted (Inserted expressed sequence, Recombinase) Mutation:    Insertion   Cd209btm1.1(cre,HBEGF)Bcgen expresses 1 gene Knock-in expresses: Organism Expressed Gene Homolog in Mouse Note human HBEGF (1839) Hbegf  (MGI:96070)   Cd209btm1.1(cre,HBEGF)Bcgen expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Cd209b (MGI:1916415)   Mutation details: A cDNA construct encoding an Frt-flanked neomycin resistance cassette followed by a stop cassette was introduced before a start codon, followed by cDNA encoding Cre recombinase, and the human DTR (HBEGF) was generated. The Cre recombinase and DTR coding sequences were separated by self-cleaving 2A viral sequences to allow stoichiometric translation of both individual proteins. This construct was inserted at the ATG start codon of cd209b, effectively replacing the expression of SIGN-R1. The neomycin resistance gene and stop cassette was removed by crossing to FLPO mice. (J:288614)

Activity:	Tissue activity of this recombinase allele
Driver:	Cd209b (mouse) Summary of all recombinase alleles driven by Cd209b. Comparative matrix view of recombinase activities Alliance mouse Cd209b gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd209b Mutation:	30 strains or lines available

Original:	J:288614 Pirgova G, et al., Marginal zone SIGN-R1(+) macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):12295-12305
All:	1 reference(s)