Gene Expression Data
Assay Details
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Assay
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Reference:
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J:111643
Boras-Granic K, et al., Genetic interaction between Lef1 and Alx4 is required for early embryonic development. Int J Dev Biol. 2006;50(7):601-10
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6755135
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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anti-Pecam1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
6A:
embryonic day 9.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: blood vessel |
Present |
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6A
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Expressed in the endothelial cells in developed vasculature.
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Specimen
6B:
embryonic day 9.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: head blood vessel |
Present |
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6B
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Expressed in the endothelial cells in a well-developed network of vessels.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Alx4lst/Alx4lst
Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: blood vessel |
Present |
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6C
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Expressed in the endothelial cells in developed vasculature. Abnormalities of the intersomitic blood vessels or the vessels of the limb buds were not observed.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Alx4lst/Alx4lst
Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: head blood vessel |
Present |
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6D
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Expressed in the endothelial cells. The vessels were smaller and there was an absence of the major vessels evident in wild type enbryos. The vascular network appeared finer and, overall, vessels were irregular in diameter and shape. The vascular network appeared finer and, overall, vessels were irregular in diameter and shape.
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Analysis Tools
