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Gene Expression Data
Assay Details
Assay
Reference: J:304555 Zamfirescu RC, et al., mTORC1/2 signaling is downregulated by amino acid-free culture of mouse preimplantation embryos and is only partially restored by amino acid readdition. Am J Physiol Cell Physiol. 2021 Jan 1;320(1):C30-C44
Assay type: Western blot
MGI Accession ID: MGI:6754123
Gene symbol: Pdpk1
Gene name: 3-phosphoinositide dependent protein kinase 1
Antibody: Phospho-PDK1 (Ser241) #3061
Assay notes: The antibody used recognizes the phosphorylated form of the protein.
Results Image: 2A P-PDK1S241
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
O P adult TS28: oocyte Present Quackenbush Swiss Female
1C E0.5 TS1: 1-cell stage conceptus Present Quackenbush Swiss Not Specified
2C E1.0 TS2: 2-cell stage conceptus Present Quackenbush Swiss Not Specified
4C E2.0 TS3: 4-cell stage conceptus Present Quackenbush Swiss Not Specified
8C E2.5 TS3: 8-cell stage conceptus Present Quackenbush Swiss Not Specified
MO E3.0 TS3: morula-stage conceptus Present Quackenbush Swiss Not Specified
BL E3.5 TS4: blastocyst-stage conceptus Weak (a) Quackenbush Swiss Not Specified
Notes:
(a) There was an overall trend of reduced phosphorylation throughout development.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory