Eif4ebp1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:304555 Zamfirescu RC, et al., mTORC1/2 signaling is downregulated by amino acid-free culture of mouse preimplantation embryos and is only partially restored by amino acid readdition. Am J Physiol Cell Physiol. 2021 Jan 1;320(1):C30-C44
Assay type:	Western blot
MGI Accession ID:	MGI:6754111
Gene symbol:	Eif4ebp1
Gene name:	eukaryotic translation initiation factor 4E binding protein 1

Antibody:	anti-Phospho-4E-BP1 (Thr37/46) #2855
Assay notes:	The antibody used recognizes the phosphorylated form of the protein.

Results

Image: 1B P-4E-BP1T37/46

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
O	P adult	TS28: oocyte	Present (a)	Quackenbush Swiss		Female
1C	E0.5	TS1: 1-cell stage conceptus	Present	Quackenbush Swiss		Not Specified
2C	E1.0	TS2: 2-cell stage conceptus	Present	Quackenbush Swiss		Not Specified
4C	E2.0	TS3: 4-cell stage conceptus	Present	Quackenbush Swiss		Not Specified
8C	E2.5	TS3: 8-cell stage conceptus	Present	Quackenbush Swiss		Not Specified
MO	E3.0	TS3: morula-stage conceptus	Present	Quackenbush Swiss		Not Specified
BL	E3.5	TS4: blastocyst-stage conceptus	Strong (b)	Quackenbush Swiss		Not Specified

Notes:
(a) This band had an increase in apparent molecular weight, consistent with phosphorylation at multiple sites. Expression was greater in the oocyte compared with the two-cell stage.
(b) This band had an increase in apparent molecular weight, consistent with phosphorylation at multiple sites.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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