Gene Expression Data
Assay Details
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Assay
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Reference:
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J:266234
Wylie LA, et al., Developmental SMAD6 loss leads to blood vessel hemorrhage and disrupted endothelial cell junctions. Dev Biol. 2018 Oct 15;442(2):199-209
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6753279
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Gene symbol:
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Smad6
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Gene name:
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SMAD family member 6
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
Present |
Regionally restricted |
2C
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Expression in descending aorta
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: dorsal aorta |
Present |
Regionally restricted |
2D
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Expression in descending aorta
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| TS21: artery |
Present |
Regionally restricted |
2D
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Expression in branchial arch arteries.
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2K:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2M:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2N:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2O:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2P:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2Q:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Absent |
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2Q
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No expression in cartilage of tibia.
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Specimen
2R:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2S:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2T:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2U:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Specimen
2V'',V''',V'''':
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: light blue - Smad6 reporter; black - SMA (Acta2 assay MGI:6753287).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: aorta |
Present |
Regionally restricted |
2V''''
,
2V''
,
2V''' inset
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2V'''
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Co-localization with Pecam1 in endothelial cells. No overlap with SMA (Acta2) or NG2 (Cspg4) expression.
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Specimen
2W'',W''',W'''':
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Smad6tm1Glvn/Smad6tm1Glvn
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: light blue - Smad6 reporter; black - SMA (Acta2 assay MGI:6753287).
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)
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