Antibody:	ADK antibody (A304-280A)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Ubiquitous	1A	Strong, ubiquitous expression throughout cerebral cortex with relatively uniform staining over soma and dendrites, as well as glia.

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present	Widespread	1B
TS28: cerebellum Purkinje cell layer	Strong		1B
TS28: cerebellum internal granule cell layer	Strong		1B
TS28: cerebellum molecular layer	Present		1B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Ubiquitous	1C
TS28: cerebellum	Strong		1C
TS28: deep cerebellar nucleus	Strong		1C
TS28: dentate gyrus	Strong		1C
TS28: main olfactory bulb	Strong		1C
TS28: brain white matter	Present	Scattered	1C	Expression in scattered cells in white matter.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong	Regionally restricted	3A	Strong nuclear expression shown as dark punctate staining of cells.
TS24: cerebral cortex ventricular layer	Strong	Regionally restricted	3A	Strong nuclear expression shown as dark punctate staining of cells.
TS24: cortical plate	Strong	Regionally restricted	3A	Strong nuclear expression shown as dark punctate staining of cells.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted	3B	Most of nuclear expression in layers II, III and IV of neocortex.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Regionally restricted	3C	Intense nuclear expression gradually declined from P2 and P5. Expression maintained in layers III and IV of neocortex.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Widespread	3D	Nuclear expression in reduced, while diffuse staining consistent with cytoplasmic expression is widespread.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong	Regionally restricted	3E	Strong nuclear expression shown as dark punctate staining of cells.
TS24: cortical plate	Strong	Regionally restricted	3E	Strong nuclear expression shown as dark punctate staining of cells.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present	Regionally restricted	3F	Most of nuclear expression in layers II, III and IV of neocortex.
TS27: cortical layer III	Present	Regionally restricted	3F	Most of nuclear expression in layers II, III and IV of neocortex.
TS27: cortical layer IV	Present	Regionally restricted	3F	Most of nuclear expression in layers II, III and IV of neocortex.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present	Regionally restricted	3G	Nuclear expression only observed in layers III and IV.
TS28: cortical layer IV	Present	Regionally restricted	3G	Nuclear expression only observed in layers III and IV.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present	Regionally restricted		Expression continued to disappear even in layer III of cortex.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present	Regionally restricted	3H	Nuclear expression in reduced, while diffuse staining consistent with cytoplasmic expression is widespread.

Structure	Level	Pattern	Image	Note
TS27: cerebellum internal granule cell layer	Present	Widespread	4A (left)

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Punctate	4A (right)	Dark, punctate expression in external granular layer, Purkinje layer and internal granular layer.
TS27: cerebellum external granule cell layer	Present	Punctate	4A (right)	Dark, punctate expression in external granular layer, Purkinje layer and internal granular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum layer	Present	Regionally restricted	4B (left)	Expression in distinct layers of cerebellar cortex.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		4B (right)
TS27: cerebellum external granule cell layer	Present		4B (right)
TS27: cerebellum internal granule cell layer	Present		4B (right)
TS27: cerebellum molecular layer	Weak		4B (right)	Expression declines in cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Weak		4C (left)	Expression declines in cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Weak	Punctate	4C (right)	Lower levels of dark, punctate expression in external granular layer, Purkinje layer and molecular layer.
TS28: cerebellum external granule cell layer	Weak	Punctate	4C (right)	Lower levels of dark, punctate expression in external granular layer, Purkinje layer and molecular layer.
TS28: cerebellum molecular layer	Weak	Punctate	4C (right)	Lower levels of dark, punctate expression in external granular layer, Purkinje layer and molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Weak		4D (left)	Expression declines in cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Weak	Punctate	4D (right)	Lower levels of dark, punctate expression in Purkinje layer and molecular layer.
TS28: cerebellum molecular layer	Weak	Punctate	4D (right)	Lower levels of dark, punctate expression in Purkinje layer and molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Regionally restricted	4E (left)	Dark punctate expression consistent with ADK-L scattered in molecular layer. Diffuse expression of ADK-S extensively observed in molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Scattered	4E (right)	Dark punctate expression consistent with ADK-L scattered in Purkinje layer and internal granule layer.
TS28: cerebellum internal granule cell layer	Present	Scattered	4E (right)	Dark punctate expression consistent with ADK-L scattered in Purkinje layer and internal granule layer.