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Gene Expression Data
Assay Details
Assay
Reference: J:306235 Allou L, et al., Non-coding deletions identify Maenli lncRNA as a limb-specific En1 regulator. Nature. 2021 Apr;592(7852):93-98
Assay type: RT-PCR
MGI Accession ID: MGI:6740434
Gene symbol: En1
Gene name: engrailed 1
Probe: En1-pE, En1-pP
Assay notes: Quantitative RT-PCR. Relative expression was normalized to wild type expression. Multiple En1 primer sets for quantitative RT-PCR were described in this paper, but the one used in this assay was not detailed. En1 primers MGI:3841732, MGI:6740408, MGI:6740410 or MGI:6740412 may have been used in this assay.
Results Image: S2e
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Limbs Wild type E11.5 TS19: limb Present Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6NCrl Not Specified
Limbs Del(27) E11.5 TS19: limb Absent (a) Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6NCrl * CD-1 Del(1)1Smun/Del(1)1SmunNot Specified
Somites Wild type E11.5 TS19: somite Present Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6NCrl Not Specified
Somites Del(27) E11.5 TS19: somite Present (b) Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6NCrl * CD-1 Del(1)1Smun/Del(1)1SmunNot Specified
Hindbrains Wild type E11.5 TS19: hindbrain Present Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6NCrl Not Specified
Hindbrains Del(27) E11.5 TS19: hindbrain Present (b) Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6NCrl * CD-1 Del(1)1Smun/Del(1)1SmunNot Specified
Notes:
(a) Authors report complete loss of expression.
(b) Expression unchanged compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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