Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:306235
Allou L, et al., Non-coding deletions identify Maenli lncRNA as a limb-specific En1 regulator. Nature. 2021 Apr;592(7852):93-98
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6740428
|
|
Gene symbol:
|
En1
|
|
Gene name:
|
engrailed 1
|
|
Probe:
|
En1 probe38
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
1e Wild type embryo:
embryonic day 11.5
(more  )
Specimen
1e Wild type embryo:
(close  )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1e Wild type limb:
embryonic day 11.5
(more )
Specimen
1e Wild type limb:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: apical ectodermal ridge |
Present |
Regionally restricted |
1e Wild type limb
|
Expression in ventral apical ectodermal ridge (AER) and ectoderm.
|
| TS19: limb ectoderm |
Present |
Regionally restricted |
1e Wild type limb
|
Expression in ventral apical ectodermal ridge (AER) and ectoderm.
|
Specimen
1e Del(27) embryo:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl * CD-1
|
|
Mutant Allele(s):
|
Del(1)1Smun/Del(1)1Smun
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1e Del(27) limb:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl * CD-1
|
|
Mutant Allele(s):
|
Del(1)1Smun/Del(1)1Smun
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Absent |
|
1e Del(27) limb
|
|
Specimen
S7a En1 Wild type:
embryonic day 11.5
(more )
Specimen
S7a En1 Wild type:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
|
S7a En1 Wild type
|
|
Specimen
S7a En1 pAS/pAS:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl * CD-1
|
|
Mutant Allele(s):
|
Gm29348em1Smun/Gm29348em1Smun
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Trace |
|
S7a En1 pAS/pAS
|
Almost complete lost of expression.
|
|
Analysis Tools