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Gene Expression Data
Assay Details
Assay
Reference: J:306155 Goodyer WR, et al., Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution. Circ Res. 2019 Aug 2;125(4):379-397
Assay type: RNA in situ
MGI Accession ID: MGI:6730335
Gene symbol: Hcn4
Gene name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Probe: Mm-Hcn4 (421271)
Probe preparation: Antisense labelled with fluorescein RNA
Visualized with: Fluorescence
Results
Specimen 3F: postnatal day 0 (more )
Note: Double labeled: red - Hcn4; green - Smoc2 (assay MGI:6740242).
Structure Level Pattern Image Note
TS27: sinoatrial node Present 3F1 , 3F4 , 3F2 Expressed within the compact sinoatrial node, but not in the surrounding atrial working myocardium.
TS27: atrium cardiac muscle Absent 3F1 , 3F4 , 3F2
Specimen 4H: embryonic day 16.5 (more )
Note: Double labeled: red - Hcn4; green - Cpne5 (assay MGI:6730334).
Structure Level Pattern Image Note
TS24: atrioventricular bundle Present Regionally restricted 4H upper right , 4H upper left , 4H lower right Co-expressed with Cpne5 in the His bundle and left bundle branch.
Specimen 7G: postnatal day 0 (more )
Note: Double labeled: red - Hcn4; green - Ntm (assay MGI:6730338).
Structure Level Pattern Image Note
TS27: purkinje fiber Present 7G2 , 7G1 , 7G4 Expressed in the immature purkinje fiber cells.
TS27: ventricle cardiac muscle Absent 7G4 , 7G2 , 7G1
Specimen S5E: postnatal day 0 (more )
Note: Double labeled: red - Hcn4; green - Ntm (assay MGI:6730338).
Structure Level Pattern Image Note
TS27: sinoatrial node Present S5E2 , S5E1 , S5E4 Expressed in the compact sinoatrial node.
Specimen S8D: postnatal day 0 (more )
Note: Double labeled: red - Hcn4; teal - Ntm (assay MGI:6730371).
Structure Level Pattern Image Note
TS27: atrioventricular node Present S8D4 , S8D2 , S8D1
TS27: impulse conducting system Present Regionally restricted S8D1 , S8D4 , S8D2 Expressed in the internodal tracks.
Specimen S10B: postnatal day 0 (more )
Note: Double labeled: red - Hcn4; green - Cpne5 (assay MGI:6730334).
Structure Level Pattern Image Note
TS27: purkinje fiber Present S10B4 , S10B1 , S10B2
TS27: ventricle cardiac muscle Absent S10B1 , S10B2 , S10B4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory