Gene Expression Data
Assay Details
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Assay
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Reference:
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J:167831
Moffatt P, et al., Hyaluronan production by means of Has2 gene expression in chondrocytes is essential for long bone development. Dev Dyn. 2011 Feb;240(2):404-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6730070
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Gene symbol:
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Ifitm5
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Gene name:
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interferon induced transmembrane protein 5
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Antibody:
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anti-Bril
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
6A:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6 * SJL
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Mutant Allele(s):
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Has2tm1.1Yama/Has2tm1.1Yama
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Present |
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6A
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Expressed in osteoblasts derived from the periosteum surrounded the diaphysis of the bone and extended around the hypertrophic zones of the adjacent growth plate.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6 * SJL
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Mutant Allele(s):
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Has2tm1.1Yama/Has2tm1.1Yama
Tg(Col2a1-cre)1Bhr/0
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Present |
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6B
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The osteoblast-containing tissue derived from the periosteum appeared thicker than in the controls and surrounded all the hypertrophic chondrocytes that were forming the primary center of ossification but did not extend toward the epiphyseal cartilage.
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