Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Atoh1em1(flpo)Hlai
Name:	atonal bHLH transcription factor 1; endonuclease-mediated mutation 1, Helen C Lai
MGI ID:	MGI:6729714
Synonyms:	Atoh1P2A-FLPo
Gene:	Atoh1  Location: Chr6:64706109-64708229 bp, + strand  Genetic Position: Chr6, 30.03 cM
Alliance:	Atoh1em1(flpo)Hlai page

Strain of Origin:

C57BL/6N

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Atoh1em1(flpo)Hlai expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Atoh1 (MGI:104654)   Mutation details: CRISPR/cas9 genome editing is used to insert a P2A, a FlpO recombinase gene after the last amino acid codon and before the stop codon of Atoh1. The cassette contained a minor mutation at the end of FLPo (the last isoleucine amino acid was changed to a serine), which could have occurred possibly because of an imprecise DNA repair event. But, this amino acid change did not affect the enzymatic function of FLPo. (J:308450)

Activity:	Tissue activity of this recombinase allele
Driver:	Atoh1 (mouse) Summary of all recombinase alleles driven by Atoh1. Comparative matrix view of recombinase activities Alliance mouse Atoh1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Atoh1 Mutation:	36 strains or lines available

Original:	J:308450 Ogujiofor OW, et al., An Atoh1 CRE Knock-In Mouse Labels Motor Neurons Involved in Fine Motor Control. eNeuro. 2021 Jan-Feb;8(1):ENEURO.0221-20.2021
All:	1 reference(s)