Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63357
Bhatt AJ, et al., Expression of vascular endothelial growth factor and Flk-1 in developing and glucocorticoid-treated mouse lung. Pediatr Res. 2000 May;47(5):606-13
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6728882
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Gene symbol:
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Vegfa
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Gene name:
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vascular endothelial growth factor A
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Probe:
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VEGF cDNA8
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4A:
embryonic day 18.0
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
4A
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Expressed in the cuboidal epithelial cells of distal airspaces.
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| TS26: lung mesenchyme |
Present |
Regionally restricted |
4A
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A relatively low expression in mesenchymal tissue between airspaces.
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Specimen
4B:
postnatal day 2
(more )
Specimen
4B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: alveolus epithelium |
Present |
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4B
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| TS27: alveolar wall |
Present |
Regionally restricted |
4B
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Expressed mainly in the alveolar epithelium, and little message was detected in mesenchymal cells, vascular smooth muscle cells, or airway cells.
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Specimen
4C:
postnatal day 8
(more )
Specimen
4C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: alveolus epithelium |
Present |
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4C
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| TS28: alveolar wall |
Present |
Regionally restricted |
4C
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Expressed mainly in the alveolar epithelium, and little message was detected in mesenchymal cells, vascular smooth muscle cells, or airway cells.
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Specimen
4D:
postnatal day 8
(more )
Specimen
4D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: alveolus epithelium |
Present |
Regionally restricted |
4D
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Expressed in rounded alveolar epithelial cells located at alveolar corners, suggestive of type II alveolar epithelial cells. No appreciable message was seen in flattened alveolar epithelial cells.
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Specimen
5A:
postnatal day 10
(more )
Specimen
5A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: alveolus epithelium |
Present |
Regionally restricted |
5A
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Expressed primarily in the distal alveolar epithelium.
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Analysis Tools