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Gene Expression Data
Assay Details
Assay
Reference: J:60699 Ogawa H, et al., Relationship of gap junction formation to phosphorylation of connexin43 in mouse preimplantation embryos. Mol Reprod Dev. 2000 Apr;55(4):393-8
Assay type: Western blot
MGI Accession ID: MGI:6728066
Gene symbol: Gja1
Gene name: gap junction protein, alpha 1
Antibody: anti-Cx43
Detection system: Secondary antibody coupled to alkaline phosphatase
Assay notes: Data was quantified in Fig 3B.
Results
Image: 3A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 43 kDa (a) 42 kDa (b) 41 kDa (c) Genetic BackgroundMutant Allele(s)Sex Note
IAR20 Cultured cells: no data stored
AP Treated IAR20 Cultured cells: no data stored
4C6H E2.0 TS3: 4-cell stage embryo Weak Weak Present involves: C3H/He * C57BL/6 * ICR Not Specified 6 hr postdivision to the four-cell stage
8C0H E2.5 TS3: 8-cell stage embryo Weak Weak Present involves: C3H/He * C57BL/6 * ICR Not Specified 0 hr postdivision to the eight-cell stage
8C6H E2.5 TS3: 8-cell stage embryo Weak Weak Present involves: C3H/He * C57BL/6 * ICR Not Specified 6 hr postdivision to the eight-cell stage
8C9H E2.5 TS3: 8-cell stage embryo Present Present Present involves: C3H/He * C57BL/6 * ICR Not Specified 9 hr postdivision to the eight-cell stage
8C12H E3.0 TS3: 8-cell stage embryo Present Present Present involves: C3H/He * C57BL/6 * ICR Not Specified 12 hr postdivision to the eight-cell stage
8C15H E3.0 TS3: 8-cell stage embryo Present Present Present involves: C3H/He * C57BL/6 * ICR Not Specified 15 hr postdivision to the eight-cell stage
Blast E3.5 TS4: blastocyst Strong Strong Strong involves: C3H/He * C57BL/6 * ICR Not Specified blastocysts
Notes:
(a) Cx43-P2: phosphorylated form 2.
(b) Cx43-P1: phosphorylated form 1.
(c) Cx43-NP: non-phosphorylated form.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
09/30/2025
MGI 6.24
The Jackson Laboratory