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Gene Expression Data
Assay Details
Assay
Reference: J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6727341
Gene symbol: Rad51
Gene name: RAD51 recombinase
Antibody: anti-RAD51
Detection system: Alexa Fluor 488
Results
Specimen 4H: postnatal day 25 (more )
Note: Leptotene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4H
Specimen 4I: postnatal day 25 (more )
Note: Zygotene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4I
Specimen 4J: postnatal day 25 (more )
Note: Pachytene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4J
Specimen 4K: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Leptotene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4K Number of foci of expression comparable to that of +/+ spermatocytes at leptotene stage.
Specimen 4L: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Zygotene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4L Number of foci of expression comparable to that of +/+ spermatocytes at early zygotene stage.
Specimen Late zygotene not shown: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted
Specimen 4M: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene-like. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4M Significantly higher number of foci of expression in spermatocytes at pachytene-like stages compared to +/+ spermatocytes.
Specimen 5M: embryonic day 17.5 (more )
Note: Zygotene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present 5M
Specimen 5N: embryonic day 17.5; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Zygotene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present Regionally restricted 5N Numbers of foci of expression comparable to +/+ oocytes at zygotene stage.
Specimen 5O: embryonic day 17.5 (more )
Note: Pachytene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present 5O
Specimen 5P: embryonic day 17.5; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene stage. Double labeled: green - Rad51; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present Regionally restricted 5P Numbers of foci of expression comparable to +/+ oocytes at pachytene stage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory