Gene Expression Data
Assay Details
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Assay
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Reference:
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J:185870
Hazen VM, et al., BMP receptor-activated Smads confer diverse functions during the development of the dorsal spinal cord. Dev Biol. 2012 Jul 15;367(2):216-27
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6727122
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Gene symbol:
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Smad9
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Gene name:
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SMAD family member 9
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Antibody:
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Anti-Phospho-Smad1/5/8
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Detection system:
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Secondary antibody coupled to Cy5
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Assay notes:
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The specificity of the antibody used was not detailed; all family members have been annotated. The antibody used recognizes the phosphorylated form of the protein.
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Results
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Specimen
1J/K:
embryonic day 10.5
(more  )
Note: Double labeled: red - Smad1, Smad5, and Smad9; green - Cntn2 (assay MGI:6727044).
Specimen
1J/K:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Smad1, Smad5, and Smad9; green - Cntn2 (assay MGI:6727044).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
1K
,
1J
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Expressed in the most dorsal progenitor cells immediately adjacent to the roof plate.
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Specimen
1L/M:
embryonic day 11.5
(more )
Note: Double labeled: red - Smad1, Smad5, and Smad9; green - Cntn2 (assay MGI:6727044).
Specimen
1L/M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Smad1, Smad5, and Smad9; green - Cntn2 (assay MGI:6727044).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube lateral wall |
Present |
Graded |
1M
,
1L
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Expanded in the ventricular zone of the dorsal-most spinal cord, with the highest levels of activation seen in the cells flanking the roof plate. Expressed in both post-crossing axons and Cntn2+ commissural axons.
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Specimen
S2A:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Smad1tm1Abr/Smad1tm1Abr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
S2A
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Expressed in the most dorsal progenitor cells immediately adjacent to the roof plate.
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Specimen
S2B:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
S2B
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Expressed in the most dorsal progenitor cells immediately adjacent to the roof plate, similar as in the control.
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Specimen
S2C:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Smad1tm1Abr/Smad1tm1Abr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
S2C
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Expressed in the commissural axons before and after crossing the floor plate.
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Specimen
S2D:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
S2D
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Substantial decrease (40%) in expression in the commissural axons before and after crossing the floor plate compared with the control.
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Specimen
S2E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Smad5tm1Huy/Smad5tm1Huy
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
S2E
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Expressed in the most dorsal progenitor cells immediately adjacent to the roof plate.
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Specimen
S2F:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
S2F
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Decreased expression (20%) in the most dorsal progenitor cells immediately adjacent to the roof plate, compared with the control.
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Analysis Tools