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Gene Expression Data
Assay Details
Assay
Reference: J:287761 Pineault KM, et al., Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life. Nat Commun. 2019 Jul 18;10(1):3168
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6724551
Gene symbol: Hoxa11
Gene name: homeobox A11
Reporter: GFP
Antibody: anti-GFP (ab13970)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1a: embryonic day 13.5; Hoxa11tm1Dmwe/Hoxa11+ (more )
Note: Double labeled: green - Hoxa11 reporter; red - Sox9 (assay MGI:6724561).
Structure Level Pattern Image Note
TS21: forearm rest of mesenchyme Present Regionally restricted 1a inset , 1a Expression is seen in cells of the perichondrium surrounding the radius/ulna chondrocyte anlage.
TS21: perichondrium Present Regionally restricted 1a inset , 1a Expression is seen in cells of the perichondrium surrounding the radius/ulna chondrocyte anlage.

Specimen 1b: embryonic day 14.5; Hoxa11tm1Dmwe/Hoxa11+ (more )
Note: Double labeled: green - Hoxa11 reporter; magenta - Sp7 (assay MGI:6724558).
Structure Level Pattern Image Note
TS22: periosteum Present Regionally restricted 1b inset , 1b Expression is seen in the outer periosteum, immediately adjacent to the differentiating osteoblast layer.
TS22: radius cartilage condensation Present Regionally restricted 1b inset , 1b Expression is seen in the outer periosteum, immediately adjacent to the differentiating osteoblast layer.
TS22: ulna cartilage condensation Present Regionally restricted 1b inset , 1b Expression is seen in the outer periosteum, immediately adjacent to the differentiating osteoblast layer.

Specimen 1c: embryonic day 18.5; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS26: periosteum Present Regionally restricted 1c Expression is seen in the perichondrium as well as on endosteal bone surfaces.
TS26: radius Present Regionally restricted 1c Expression is seen in the perichondrium as well as on endosteal bone surfaces.
TS26: ulna Present Regionally restricted 1c Expression is seen in the perichondrium as well as on endosteal bone surfaces.

Specimen 1d: postnatal week 2; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS28: radius Present Regionally restricted 1d Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: periosteum Present Regionally restricted 1d , 1d inset Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 1d , 1d inset Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: bone marrow Present Regionally restricted 1d inset , 1d Expression is seen in stromal cells within the bone marrow space.

Specimen 1e: postnatal week 8; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS28: radius Present Regionally restricted 1e Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: periosteum Present Regionally restricted 1e inset , 1e Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 1e inset , 1e Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: bone marrow Present Regionally restricted 1e , 1e inset Expression is seen in stromal cells within the bone marrow space.

Specimen 1f: postnatal month 10; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS28: radius Present Regionally restricted 1f Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: periosteum Present Regionally restricted 1f , 1f inset Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 1f , 1f inset Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: bone marrow Present Regionally restricted 1f , 1f inset Expression is seen in stromal cells within the bone marrow space.

Specimen 1g: postnatal week 8; Hoxa11tm1Dmwe/Hoxa11+ (more )
Note: Double labeled: green - Hoxa11 reporter; red - Sp7 (assay MGI:6724558).
Structure Level Pattern Image Note
TS28: long bone Present Regionally restricted 1g green , 1g merge Expression is seen on bone surfaces.

Specimen 1h - E15.5: embryonic day 15.5; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS23: long bone epiphyseal plate Present Regionally restricted 1h E15.5 Expression is seen in the distal growth plate.
TS23: perichondrium Present 1h E15.5 inset , 1h E15.5

Specimen 1h - 8 weeks: postnatal week 8; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS28: periosteum Present 1h 8 weeks

Specimen 1h - P4: postnatal day 4; Hoxa11tm1Dmwe/Hoxa11+ (more )
Structure Level Pattern Image Note
TS28: trabecular bone Present 1h P4

Specimen 1i: postnatal week 2; Hoxa11tm1Dmwe/Hoxa11+ (more )
Note: Double labeled: green - Hoxa11 reporter; red - Postn (assay MGI:6724559).
Structure Level Pattern Image Note
TS28: periosteum Present Non-Uniform 1i green , 1i merge The expression is seen in the outer and inner periosteum; it is weaker in the inner periosteal layer.

Specimen 1j: postnatal week 8; Hoxa11tm1Dmwe/Hoxa11+ (more )
Note: Double labeled: green - Hoxa11 reporter; red - Postn (assay MGI:6724559).
Structure Level Pattern Image Note
TS28: periosteum Present Non-Uniform 1j green , 1j merge The expression is seen in the outer and inner periosteum; it is weaker in the inner periosteal layer.

Specimen 7a: embryonic day 18.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS26: forelimb bone Present Regionally restricted 7a Expression is seen through the periosteum and along bone surfaces of the primary spongiosa.
TS26: periosteum Present Regionally restricted 7a Expression is seen through the periosteum and along bone surfaces of the primary spongiosa.

Specimen 7b: postnatal week 2; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: bone marrow Present Single cells 7b Expression is seen in stromal cells in the bone marrow space.
TS28: ulna Present Single cells 7b Expression is seen in stromal cells in the bone marrow space.

Specimen 7b': postnatal week 2; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: periosteum Present Regionally restricted 7b' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 7b' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.

Specimen 7c: postnatal week 4; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: bone marrow Present Single cells 7c Expression is seen in stromal cells in the bone marrow space.
TS28: ulna Present Single cells 7c Expression is seen in stromal cells in the bone marrow space.

Specimen 7c': postnatal week 4; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: periosteum Present Regionally restricted 7c' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 7c' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.

Specimen 7d: postnatal week 8; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: bone marrow Present Single cells 7d Expression is seen in stromal cells in the bone marrow space.
TS28: periosteum Present Single cells 7d Expression is seen in stromal cells in the bone marrow space.

Specimen 7d': postnatal week 8; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: periosteum Present Regionally restricted 7d' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 7d' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.

Specimen 7e: postnatal week 15; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: bone marrow Present Single cells 7e Expression is seen in stromal cells in the bone marrow space.
TS28: ulna Present Single cells 7e Expression is seen in stromal cells in the bone marrow space.

Specimen 7e': postnatal week 15; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hoxa11tm1Dmwe/Hoxa11+, Leprtm2(cre)Rck/Lepr+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa11 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS28: periosteum Present Regionally restricted 7e' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.
TS28: ulna Present Regionally restricted 7e' Expression is seen on the periosteal surface as well as on endosteal bone surfaces.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory