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Gene Expression Data
Assay Details
Assay
Reference: J:299851 Sutton AK, et al., Paraventricular, subparaventricular and periventricular hypothalamic IRS4-expressing neurons are required for normal energy balance. Sci Rep. 2020 Mar 26;10(1):5546
Assay type: RNA in situ
MGI Accession ID: MGI:6721413
Gene symbol: Irs4
Gene name: insulin receptor substrate 4
Probe: Mm-Irs4 (482511)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 1B: postnatal week 8-16; Irs4tm1.1(cre)Dolsn/Y (more )
Note: Double labeled: red - Irs4 RNA; green - Irs4 reporter (assay MGI:6720264).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 1B There is substantial overlap between the reporter and mRNA expression.
Specimen S1A: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Crh (assay MGI:6721414).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1A Expression is seen in neurons; only 9.8% of them also express Crh.
Specimen S1B: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Crh (assay MGI:6721414).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1B Expression is seen in neurons; only 9.8% of them also express Crh.
Specimen S1C: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Crh (assay MGI:6721414).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1C Expression is seen in neurons; only 9.8% of them also express Crh.
Specimen S1D: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Pdyn (assay MGI:6721415).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1D Expression is seen in neurons; only 15.8% of them also express Pdyn.
Specimen S1E: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Pdyn (assay MGI:6721415).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1E Expression is seen in neurons; only 15.8% of them also express Pdyn.
Specimen S1F: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Pdyn (assay MGI:6721415).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1F Expression is seen in neurons; only 15.8% of them also express Pdyn.
Specimen S1G: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Trh (assay MGI:6721416).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1G Expression is seen in neurons; 40.5% of them also express Trh.
Specimen S1H: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Trh (assay MGI:6721416).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1H Expression is seen in neurons; 40.5% of them also express Trh.
Specimen S1I: postnatal week 8-16 (more )
Note: Double labeled: red - Irs4; green - Trh (assay MGI:6721416).
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1I Expression is seen in neurons; 40.5% of them also express Trh.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory