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Gene Expression Data
Assay Details
Assay
Reference: J:241010 Mu W, et al., EZH1 in germ cells safeguards the function of PRC2 during spermatogenesis. Dev Biol. 2017 Apr 15;424(2):198-207
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721046
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: Anti-gamma H2AX
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1I Ctrl1: postnatal month 2; Ezh2tm1Tara/Ezh2+, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 1I upper left
Specimen 1I Ctrl2: postnatal month 2; Ezh2tm1Tara/Ezh2+, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 1I lower left
Specimen 1I Mut1: postnatal month 2; Ezh2tm1Tara/Ezh2tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 1I upper right
Specimen 1I Mut2: postnatal month 2; Ezh2tm1Tara/Ezh2tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 1I lower right
Specimen 4C Ctrl1: postnatal month 1; Ezh1tm1Jnw/Ezh1+, Ezh2tm1Tara/Ezh2+, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C Ctrl-1
Specimen 4C Ctrl2: postnatal month 1; Ezh1tm1Jnw/Ezh1+, Ezh2tm1Tara/Ezh2+, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C Ctrl-2
Specimen 4C Mut1: postnatal month 1; Ezh1tm1Jnw/Ezh1tm1Jnw, Ezh2tm1Tara/Ezh2tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C DKO-1 Depletion of pachytene spermatocytes and spermatids.
Specimen 4C Mut2: postnatal month 1; Ezh1tm1Jnw/Ezh1tm1Jnw, Ezh2tm1Tara/Ezh2tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C DKO-2 Depletion of pachytene spermatocytes and spermatids.
Specimen 4D Ctrl1: postnatal day 13; Ezh1tm1Jnw/Ezh1+, Ezh2tm1Tara/Ezh2+, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:6721116).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D-1
Specimen 4D Mut1: postnatal day 13; Ezh1tm1Jnw/Ezh1tm1Jnw, Ezh2tm1Tara/Ezh2tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:6721116).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D-2 Depletion of pachytene spermatocytes and spermatids.
Specimen 4D Ctrl2: postnatal month 1; Ezh1tm1Jnw/Ezh1+, Ezh2tm1Tara/Ezh2+, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:6721116)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D-3
Specimen 4D Mut2: postnatal month 1; Ezh1tm1Jnw/Ezh1tm1Jnw, Ezh2tm1Tara/Ezh2tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:6721116).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D-4 Depletion of pachytene spermatocytes and spermatids.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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