Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Eomestm2.1(flpo/ERT2)Zjh
Name:	eomesodermin; targeted mutation 2.1, Z Josh Huang
MGI ID:	MGI:6720888
Synonyms:	Tbr2-FlpER
Gene:	Eomes  Location: Chr9:118307259-118315176 bp, + strand  Genetic Position: Chr9, 70.21 cM, cytoband F3
Alliance:	Eomestm2.1(flpo/ERT2)Zjh page

Germline Transmission:	Earliest citation of germline transmission: J:323692
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Eomestm2.1(flpo/ERT2)Zjh expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Eomes (MGI:1201683)   Mutation details: The allele was generated by inserting a 2A-flpoER expression cassette in-frame before the STOP codon of the Eomes gene. The targeting vectors generated using BAC clones from the RPCI-23&24 library were electroporated into a 129SVj/B6 hybrid ES cell line (v6.5). The Resulting ES cell clones were used for tetraploid complementation to obtain male heterozygous mice. The neo selection cassette was excised by crossing to cre expressing mice. (J:323692)

Activity:	Tissue activity of this recombinase allele
Driver:	Eomes (mouse) Summary of all recombinase alleles driven by Eomes. Alliance mouse Eomes gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Eomes Mutation:	41 strains or lines available

Original:	J:323692 Matho KS, et al., Genetic dissection of the glutamatergic neuron system in cerebral cortex. Nature. 2021 Oct;598(7879):182-187
All:	1 reference(s)