Myh1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:241564 Quinn ME, et al., Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development. Nat Commun. 2017 Jun 01;8:15665
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6720388
Gene symbol:	Myh1
Gene name:	myosin, heavy polypeptide 1, skeletal muscle, adult

Antibody:	clone MY-32 (M4276)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

6c WT (upper) ©

Specimen 6c WT (upper): embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: trunk musculature	Present	Regionally restricted	6c WT (upper)

6c Myomerger KO (upper) ©

Specimen 6c Myomerger KO (upper): embryonic day 15.5; Mymx^em1Shcs/Mymx^em1Shcs (more

)

Structure	Level	Pattern	Image	Note
TS23: trunk musculature	Present	Regionally restricted	6c Myomerger KO (upper)	Expression in muscle cells with sarcomereic structures.

6c WT (lower) ©

Specimen 6c WT (lower): embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: trunk musculature	Present	Regionally restricted	6c WT (lower)	Myofibres were multinucleated.

6c Myomerger KO (lower) ©

Specimen 6c Myomerger KO (lower): embryonic day 15.5; Mymx^em1Shcs/Mymx^em1Shcs (more

)

Structure	Level	Pattern	Image	Note
TS23: trunk musculature	Present	Regionally restricted	6c Myomerger KO (lower)	Myosin-expressing myocytes with sarcomeres remained mononucleated.

S5c WT (left) ©

Specimen S5c WT (left): embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: forelimb muscle	Present	Regionally restricted	S5c WT (left)

S5c Myomerger KO (left) ©

Specimen S5c Myomerger KO (left): embryonic day 15.5; Mymx^em1Shcs/Mymx^em1Shcs (more

)

Structure	Level	Pattern	Image	Note
TS23: forelimb muscle	Present	Regionally restricted	S5c Myomerger KO (left)	Myoblasts differentiate but are unable to fuse.

S5c WT (right) ©

Specimen S5c WT (right): embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: forelimb muscle	Present	Regionally restricted	S5c WT (right)

S5c Myomerger KO (right) ©

Specimen S5c Myomerger KO (right): embryonic day 15.5; Mymx^em1Shcs/Mymx^em1Shcs (more

)

Structure	Level	Pattern	Image	Note
TS23: forelimb muscle	Present	Regionally restricted	S5c Myomerger KO (right)	Myoblasts unable to fuse.

S5e WT ©

Specimen S5e WT: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: forelimb muscle	Present	Regionally restricted	S5e WT

Specimen S5e Myomerger KO: embryonic day 17.5; Mymx^em1Shcs/Mymx^em1Shcs (more

)

Structure	Level	Pattern	Image	Note
TS25: forelimb muscle	Present	Regionally restricted	S5e Myomerger KO	Myoblasts unable to fuse.

S5f WT ©

Specimen S5f WT: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: forelimb muscle	Present	Regionally restricted	S5f WT

Specimen S5f Myomerger KO: embryonic day 17.5; Mymx^em1Shcs/Mymx^em1Shcs (more

)

Structure	Level	Pattern	Image	Note
TS25: forelimb muscle	Present	Regionally restricted	S5f Myomerger KO	Some myoblasts contained two nuclei.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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