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Gene Expression Data
Assay Details
Assay
Reference: J:306910 Yu DCW, et al., Human pluripotent stem cell-derived DDX4 and KRT-8 positive cells participate in ovarian follicle-like structure formation. iScience. 2021 Jan 22;24(1):102003
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6719332
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5A E7.5-8: embryonic day 7.5-8.0 (more )
Note: Double labeled: green - Ddx4; red - Krt8 (assay MGI:6719333).
Structure Level Pattern Image Note
TS11: primordial germ cell Present 5A E7.5-8 Merge , 5A E7.5-8 , 5A E7.5-8 Ddx4
Specimen 5A E9.5-10: embryonic day 9.5-10.0 (more )
Note: Double labeled: green - Ddx4; red - Krt8 (assay MGI:6719333).
Structure Level Pattern Image Note
TS15: primordial germ cell Present 5A E9.5-10 Ddx4 , 5A E9.5-10 Merge , 5A E9.5-10
Specimen 5A E11.5-12: embryonic day 11.5-12.0 (more )
Note: Double labeled: green - Ddx4; red - Krt8 (assay MGI:6719333).
Structure Level Pattern Image Note
TS19: primordial germ cell Present Regionally restricted 5A E11.5-12 , 5A E11.5-12 Ddx4 , 5A E11.5-12 Merge Co-expression in only 30% of Krt8-expressing cells.
Specimen 5A E13.5-14: embryonic day 13.5-14.0 (more )
Note: Double labeled: green - Ddx4; red - Krt8 (assay MGI:6719333).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present Regionally restricted 5A E13.5-14 Merge , 5A E13.5-14 Ddx4 , 5A E13.5-14 Co-expression in less than 10% of Krt8-expressing cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory