Antibody:	anti-MET
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to DyLight 488

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Present	Regionally restricted	2g , 2d , 2j	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS24: medulla oblongata	Present	Regionally restricted	2g , 2d , 2j	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Present	Regionally restricted	2k , 2h , 2e	Expression is in subsets of cranial motor nuclei: in and lateral to the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII) and in a subpopulation of neurons within the nucleus of solitary tract.
TS24: medulla oblongata	Present	Regionally restricted	2k , 2h , 2e	Expression is in subsets of cranial motor nuclei: in and lateral to the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII) and in a subpopulation of neurons within the nucleus of solitary tract.

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Present	Regionally restricted	2i , 2f , 2l	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS24: medulla oblongata	Present	Regionally restricted	2i , 2f , 2l	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present	Regionally restricted	3d , 3c , 3f , 3a	Expression is in somata and axons in a subpopulation of ventrolaterally situated neurons within the boundary of the dorsal motor nucleus of the tenth nerve.
TS24: brainstem nucleus	Present	Regionally restricted	3d , 3c , 3f , 3a	Expression is in somata and axons in a subpopulation of ventrolaterally situated neurons within the boundary of the dorsal motor nucleus of the tenth nerve.

Structure	Level	Pattern	Image	Note
TS21: brainstem	Present	Regionally restricted	S3b , S3c	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS21: brainstem nucleus	Present	Regionally restricted	S3b , S3c	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS21: midbrain roof plate	Present	Regionally restricted	S3b , S3c	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).

Structure	Level	Pattern	Image	Note
TS21: brainstem	Present	Regionally restricted	S3e , S3f	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS21: brainstem nucleus	Present	Regionally restricted	S3e , S3f	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS21: midbrain roof plate	Present	Regionally restricted	S3e , S3f	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).

Structure	Level	Pattern	Image	Note
TS21: brainstem	Present	Regionally restricted	S3i , S3h	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS21: brainstem nucleus	Present	Regionally restricted	S3i , S3h	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS21: midbrain roof plate	Present	Regionally restricted	S3i , S3h	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).