Probe:	Extl3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: hindbrain ventricular layer	Strong	Regionally restricted	2A	Strong expression in neurons of ventricular zones surrounding lateral and fourth ventricles.
TS19: telencephalon ventricular layer	Strong	Regionally restricted	2A	Strong expression in neurons of ventricular zones surrounding lateral and fourth ventricles.
TS19: future spinal cord	Strong		2A
TS19: alimentary system epithelium	Strong		2A
TS19: liver	Strong		2A
TS19: vertebral cartilage condensation	Present		2A	Expression in cartilage models of vertebra.
TS19: heart	Absent		2A

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	2C	Authors report more or less comparable pattern of expression as that seen at E11.5.
TS20: heart	Present		2C	Clear expression in heart.
TS20: ganglionic eminence	Present		2C
TS20: dorsal root ganglion	Present	Regionally restricted	2C	Expression in neurons of dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS22: embryo	Weak		2D	Intensity of expression generally less than at previous ages.
TS22: heart	Present		2D
TS22: lung	Present		2D

Structure	Level	Pattern	Image	Note
TS24: heart	Strong		2E
TS24: lung	Strong		2E
TS24: alimentary system	Weak		2E	Expression in gastrointestinal tract, liver and kidney is less than at previous ages.
TS24: liver	Weak		2E	Expression in gastrointestinal tract, liver and kidney is less than at previous ages.
TS24: metanephros	Weak		2E	Expression in gastrointestinal tract, liver and kidney is less than at previous ages.

Structure	Level	Pattern	Image	Note
TS26: heart	Strong		2F
TS26: lung	Strong		2F
TS26: alimentary system	Weak		2F	Expression in gastrointestinal tract, liver and kidney is less than at previous ages.
TS26: liver	Weak		2F	Expression in gastrointestinal tract, liver and kidney is less than at previous ages.

Structure	Level	Pattern	Image	Note
TS19: liver	Present		3A
TS19: cardiac muscle tissue	Absent		3A

Structure	Level	Pattern	Image	Note
TS20: alimentary system epithelium	Strong		3B	Strong expression in liver and epithelium of gastrointestinal tract.
TS20: liver	Strong		3B	Strong expression in liver and epithelium of gastrointestinal tract.
TS20: metanephros	Strong		3B	Strong expression in epithelium of metanephros.
TS20: alimentary system mesenchyme	Weak		3B	Less intense expression in gastrointestinal tract mesenchymal layer.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3C	Expresssion in developing neurons of spinal cord.
TS20: vertebral cartilage condensation	Present		3C
TS20: dorsal aorta	Present	Regionally restricted	3C	Expression in endothelial lining of large blood vessels such as the aorta.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Strong		3D	Intense expression in ventricular zone facing the lateral ventricle.
TS22: cerebral cortex intermediate zone	Present		3D
TS22: cortical plate	Present		3D

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Strong	Regionally restricted	5A	Strong expression in pancreatic epithelium.
TS19: duodenum epithelium	Strong		5A	Less intense expression in gastric and duodenal epithelium and their mesenchyme.
TS19: duodenum mesenchyme	Strong		5A	Less intense expression in gastric and duodenal epithelium and their mesenchyme.
TS19: stomach epithelium	Strong		5A	Less intense expression in gastric and duodenal epithelium and their mesenchyme.
TS19: stomach mesenchyme	Strong		5A	Less intense expression in gastric and duodenal epithelium and their mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	5B	Less strong expression in developing acini. Moderate expression in developing Islets of Langerhans.

Structure	Level	Pattern	Image	Note
TS22: pancreatic acinus	Present		5C	Expression in acini more or less comparable to that of previous age.

Structure	Level	Pattern	Image	Note
TS24: pancreatic acinus	Strong			Stronger expression in acini that at previous ages; more or less comparable to that of adult.

Structure	Level	Pattern	Image	Note
TS26: pancreatic acinus	Strong		5D	Stronger expression in acini that at previous ages; more or less comparable to that of adult.
TS26: islets of Langerhans	Moderate		5D

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Strong		5E
TS28: pancreas vasculature	Weak	Regionally restricted	5E	Weak expression in interlobular blood vessels.

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Strong		5F
TS28: islets of Langerhans	Weak		5F

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Weak