Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:207611
Lange AW, et al., Sox17 is required for normal pulmonary vascular morphogenesis. Dev Biol. 2014 Mar 1;387(1):109-20
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6717404
|
|
Gene symbol:
|
Sox17
|
|
Gene name:
|
SRY (sex determining region Y)-box 17
|
|
Antibody:
|
anti-Sox17
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
|
Results
|
Specimen
1A:
embryonic day 12.5
(more  )
Note: Double labeled: green - Sox17; red - Emcn (assay MGI:6717450).
Specimen
1A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Sox17; red - Emcn (assay MGI:6717450).
|
Specimen
1B:
embryonic day 14.5
(more )
Note: Double labeled: green - Sox17; red - Emcn (assay MGI:6717450).
Specimen
1B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Sox17; red - Emcn (assay MGI:6717450).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung vascular element |
Present |
Regionally restricted |
1B
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS22: pulmonary artery |
Present |
Regionally restricted |
1B
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS22: lung epithelium |
Absent |
|
1B
|
|
Specimen
1C:
embryonic day 18.5
(more )
Note: Double labeled: green - Sox17; red - Emcn (assay MGI:6717450).
Specimen
1C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Sox17; red - Emcn (assay MGI:6717450).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung vascular element |
Present |
Regionally restricted |
1C
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS26: pulmonary artery |
Present |
Regionally restricted |
1C
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS26: lung epithelium |
Absent |
|
1C
|
|
Specimen
1D:
embryonic day 12.5
(more )
Note: Double labeled: green - Sox17; red - Pecam1 (assay MGI:6717451).
Specimen
1D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Sox17; red - Pecam1 (assay MGI:6717451).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung vascular element |
Present |
Regionally restricted |
1D
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS20: pulmonary artery |
Present |
Regionally restricted |
1D
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS20: lung epithelium |
Absent |
|
1D
|
|
Specimen
1E:
embryonic day 14.5
(more )
Note: Double labeled: green - Sox17; red - Pecam1 (assay MGI:6717451).
Specimen
1E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Sox17; red - Pecam1 (assay MGI:6717451).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung vascular element |
Present |
Regionally restricted |
1E
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS22: pulmonary artery |
Present |
Regionally restricted |
1E
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS22: lung epithelium |
Absent |
|
1E
|
|
Specimen
1F:
embryonic day 18.5
(more )
Note: Double labeled: green - Sox17; red - Pecam1 (assay MGI:6717451).
Specimen
1F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Sox17; red - Pecam1 (assay MGI:6717451).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung vascular element |
Present |
Regionally restricted |
1F
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS26: pulmonary artery |
Present |
Regionally restricted |
1F
|
Expression was more strongly detected in arterial and microvascular endothelial cells compared to veins.
|
| TS26: lung epithelium |
Absent |
|
1F
|
|
|
Analysis Tools