Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Foxp3tm4(EGFP/icre)Tch
Name:	forkhead box P3; targeted mutation 4, Talal A Chatila
MGI ID:	MGI:6717215
Synonyms:	Foxp3deltaEGFPiCre
Gene:	Foxp3  Location: ChrX:7445915-7461482 bp, + strand  Genetic Position: ChrX, 3.41 cM
Alliance:	Foxp3tm4(EGFP/icre)Tch page

Germline Transmission:	Earliest citation of germline transmission: J:305614
Parent Cell Line:	SCC10 (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted (Recombinase, Reporter) Mutation:    Insertion   Foxp3tm4(EGFP/icre)Tch expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Foxp3 (MGI:1891436)   Mutation details: The targeting construct consisting of the IRES-EGFP-iCre sequence inserted immediately downstream of the Foxp3 translational stop codon and upstream of the endogenous polyadenylation signal and additional FRT flanked PGK-neo cassette inserted between exons 9-10 was used for homologous recombination in the ES cells. The PGK-neo cassette was removed by mating founder males with Flp-deleter female mice. Inserted Frt element inserted between exons 9 and 10 that created an aberrant 3 splice junction site, leading to a premature stop codon in the Foxp3 transcript. (J:305614)

Activity:	Tissue activity of this recombinase allele
Driver:	Foxp3 (mouse) Summary of all recombinase alleles driven by Foxp3. Comparative matrix view of recombinase activities Alliance mouse Foxp3 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Foxp3 Mutation:	55 strains or lines available

Original:	J:305614 Charbonnier LM, et al., Functional reprogramming of regulatory T cells in the absence of Foxp3. Nat Immunol. 2019 Sep;20(9):1208-1219
All:	6 reference(s)