Col10a1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:236167 Li J, et al., Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required for Normal Endochondral Bone Development. Mol Cell Biol. 2016 Sep 1;36(17):2282-99
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6715263
Gene symbol:	Col10a1
Gene name:	collagen, type X, alpha 1

Antibody:	anti-Collagen type X
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 6B WT: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: humerus	Present	Regionally restricted	6B WT	Expression was detected in hypertrophic chondrocytes.

Specimen 6B fro/fro: embryonic day 16.5; Smpd3^fro/Smpd3^fro (more

)

Structure	Level	Pattern	Image	Note
TS24: humerus	Present	Regionally restricted	6B fro/fro	Expression was detected in hypertrophic chondrocytes.

Specimen 11 Smpd3^flox/flox: embryonic day 15.5; Smpd3^tm1Murs/Smpd3^tm1Murs (more

)

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	11A Smpd3^flox/flox	Expression was detected in the hypertrophic zone.

Specimen 11 Smpd3^flox/flox;Osx-Cre: embryonic day 15.5; Smpd3^tm1Murs/Smpd3^tm1Murs, Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	11A Smpd3^flox/flox;Osx-Cre	Conditional knockouts showed a decreased presence of hypertrophic chondrocytes with condensed ring-shaped or collapsed nuclei, which indicate the early stages of apoptosis.

Specimen 11 Smpd3^flox/flox;Col2a1-Cre: embryonic day 15.5; Smpd3^tm1Murs/Smpd3^tm1Murs, Tg(Col2a1-cre)1Bhr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	11A Smpd3^flox/flox;Col2a1-Cre	Conditional knockouts showed a decreased presence of hypertrophic chondrocytes with condensed ring-shaped or collapsed nuclei, which indicate the early stages of apoptosis.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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