Probe:	En1 probe37
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		2H	Expression not altered in mutant compared with wild type.
TS22: midbrain tegmentum	Present		2H	Expression not altered in mutant compared with wild type.
TS22: midbrain-hindbrain junction	Present		2H	Expression not altered in mutant compared with wild type.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		2I	Expression not altered in mutant compared with wild type.
TS22: midbrain tegmentum	Present		2I	Expression not altered in mutant compared with wild type.
TS22: midbrain-hindbrain junction	Present		2I	Expression not altered in mutant compared with wild type.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		2J	Expression in isthmic derivatives.
TS22: midbrain tegmentum	Present		2J	Expression in isthmic derivatives.
TS22: midbrain-hindbrain junction	Present		2J	Expression in isthmic derivatives.

Structure	Level	Pattern	Image	Note
TS22: tectum	Present	Regionally restricted	4C	Expression in caudal part of tectum
TS22: cerebellum	Present		4C
TS22: midbrain tegmentum	Present		4C

Structure	Level	Pattern	Image	Note
TS22: tectum	Absent		4D	Authors report loss of tectum.
TS22: midbrain tegmentum	Present	Regionally restricted	4D	Loss of part of midbrain tegmentum.
TS22: cerebellum	Absent		4D	Complete loss of cerebellum anlage.