Reporter:	lacZ
Antibody:	anti-B-galactosidase (5Prime3Prime)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: renal artery	Present		1F Branch of Renal Artery
TS28: smooth muscle layer of renal artery	Present		1F Branch of Renal Artery

Structure	Level	Pattern	Image	Note
TS28: afferent arteriole of mature renal corpuscle	Present		1F Afferent Arteriole	In the afferent arteriole, the endothelial cell expression was higher than in the renal artery. Vascular smooth muscle cells were also labeled in the afferent arteriole.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		1F Glomerulus	Endothelial cell expression was higher than in the renal artery. Glomerular mesangial cell staining was obscure.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		1H inset
TS28: distal convoluted tubule	Absent		1H inset

Structure	Level	Pattern	Image	Note
TS27: comma-shaped body	Present		3Ba
TS27: s-shaped body	Present		3Ba
TS27: visceral epithelium of maturing renal corpuscle	Present		3Ba

Structure	Level	Pattern	Image	Note
TS27: capillary loop renal corpuscle	Present		3Bb
TS27: maturing glomerular tuft	Present		3Bb	Expression was detected in glomerular endothelial, mesangial, and epithelial cells.

Structure	Level	Pattern	Image	Note
TS27: capillary loop renal corpuscle	Present		3Bc
TS27: maturing glomerular tuft	Present		3Bc	Expression was detected in glomerular endothelial, mesangial, and epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		3Bd	Expression was detected in glomerular endothelial cells in the adult.

Structure	Level	Pattern	Image	Note
TS22: renal vasculature	Present	Regionally restricted	4A EphrinB2 + CD31 , 4A top EphrinB2	At E14.5, expression was detected only in sprouting and branching arterial endothelial cells but not in surrounding smooth muscle cells.
TS22: arterial system smooth muscle	Absent		4A top EphrinB2 , 4A EphrinB2 + CD31

Structure	Level	Pattern	Image	Note
TS22: renal vasculature	Present	Regionally restricted	4A EphrinB2 + alphaSMA , 4A bottom EphrinB2	At E14.5, expression was detected only in sprouting and branching arterial endothelial cells but not in surrounding smooth muscle cells.
TS22: arterial system smooth muscle	Absent		4A bottom EphrinB2 , 4A EphrinB2 + alphaSMA

Structure	Level	Pattern	Image	Note
TS27: arterial system smooth muscle	Present	Regionally restricted	4B EphrinB2 , 4B EphrinB2 + CD31	In the neonatal stage, expression was detected in endothelial and smooth muscle cells of the renal arterial circulation.
TS27: renal vasculature	Present	Regionally restricted	4B EphrinB2 , 4B EphrinB2 + CD31	In the neonatal stage, expression was detected in endothelial and smooth muscle cells of the renal arterial circulation.

Structure	Level	Pattern	Image	Note
TS28: artery smooth muscle	Present	Regionally restricted	4C EphrinB2 + CD31 , 4C EphrinB2	In the adult, expression was dominantly in smooth muscle cells of the renal arterial circulation. Endothelial cell expression was reduced.
TS28: renal vasculature	Present	Regionally restricted	4C EphrinB2 + CD31 , 4C EphrinB2	In the adult, expression was dominantly in smooth muscle cells of the renal arterial circulation. Endothelial cell expression was reduced.