Probe:	P1011AS
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Level	Pattern	Image	Note
TS28: uterus	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		3A	Expression in aorta.
TS20: cardiac muscle tissue	Weak		3A	Low level expression in myocardium
TS20: outflow tract	Strong	Regionally restricted	3A	Prominent expression in cardiac outflow tract, particularly root of aorta.

Structure	Level	Pattern	Image	Note
TS20: common dorsal aorta	Present	Regionally restricted	3D	Expression in abdominal aorta.
TS20: mesenchyme of metanephros	Present	Regionally restricted	3D	Expression in differentiating mesenchyme in vicinity of prospective kidneys.

Structure	Level	Pattern	Image	Note
TS20: common dorsal aorta	Present	Regionally restricted	3D inset	Expression in smooth muscle cells of prospective arterial tunica media. No expression in endothelial layers.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Diffuse		Diffuse expression throughout craniofacial mesenchyme.
TS20: mandibular process mesenchyme	Strong	Regionally restricted		Higher expression in mesenchymal cells surrounding cartilage condensations for Meckel's cartilage and the clavicle.
TS20: trunk mesenchyme	Strong	Regionally restricted		Higher expression in mesenchymal cells surrounding cartilage condensations for Meckel's cartilage and the clavicle.
TS20: nasal epithelium	Present
TS20: liver	Present	Regionally restricted		Expression in liver capsule.

Structure	Level	Pattern	Image	Note
TS25: umbilical artery	Present		3E

Structure	Level	Pattern	Image	Note
TS25: umbilical artery	Present	Regionally restricted	3E inset	Expression in smooth muscle cells of prospective arterial tunica media. No expression in endothelial layers.

Structure	Level	Pattern	Image	Note
TS25: shoulder mesenchyme	Strong	Regionally restricted	4A	Strong expression in tendons around shoulder joint.
TS25: humerus	Present	Regionally restricted	4A	Expression in metaphyseal region immediately adjacent to hypertrophic zone.
TS25: perichondrium	Strong	Regionally restricted	4A	Prominent expression in perichondrium; peak expression at point of transition of chondrocytes from small to hypertrophic cells.

Structure	Level	Pattern	Image	Note
TS25: humerus	Present	Regionally restricted	4A inset	Expression in metaphysis at site of hypertrophic cartilage resorption.

Structure	Level	Pattern	Image	Note
TS25: cruciate ligament of knee	Strong	Regionally restricted	6A	SVery prominent expression in anterior cruciate ligament and posterior cruciate ligament.
TS25: patellar ligament	Present		6A	Expression in patellar tendon.
TS25: femur	Present	Regionally restricted	6A	Expression in superfical layers of articular cartilage.
TS25: tibia	Present	Regionally restricted	6A	Expression in superfical layers of articular cartilage.

Structure	Level	Pattern	Image	Note
TS25: trigeminal ganglion	Absent
TS25: vestibulo-cochlear ganglion	Absent
TS25: hip joint	Strong	Regionally restricted		Strong expression in joint capsule of hip.
TS25: penis	Present	Regionally restricted		Expression in cells in bulbospongiosus and corpora cavernosa of developing penis.

Structure	Level	Pattern	Image	Note
TS22: umbilical artery	Present	Regionally restricted	7A	Expression in smooth muscle cells of prospective arterial tunica media. No expression in endothelial layers.
TS22: bladder	Present	Regionally restricted	7A	Expression in muscular wall of urinary bladder.

Structure	Level	Pattern	Image	Note
TS22: lower urinary tract	Present	Regionally restricted	7B	Expression in cells in and around the bulbourethral gland, adjacent to the bulbar urethra.

Structure	Level	Pattern	Image	Note
TS20: spongiotrophoblast	Present		8A	Expression in junctional area that includes decidual reaction of uterus and spongiotrophoblast of placenta.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		8A	Expression in junctional area that includes decidual reaction of uterus and spongiotrophoblast of placenta.