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Gene Expression Data
Assay Details
Assay
Reference: J:241850 Naiman N, et al., Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development. Dev Cell. 2017 May 22;41(4):349-365.e3
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6711811
Gene symbol: Pax2
Gene name: paired box 2
Antibody: anti-Pax2 (ab37129)
Detection system: Secondary antibody coupled to DyLight
Assay notes: The epitope of the antibody used in this assay is absent from the translated product of the deletion allele.
Results
Specimen 1M/O: embryonic day 12.5; Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Pax2; green - Cre; white - cytokeratin
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 1M , 1O
TS20: nephron Present 1M , 1O Expression is present in differentiating nephrons.
TS20: ureteric tip Present 1M , 1O
Specimen 1N/P: embryonic day 12.5; Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Pax2; green - Cre; white - cytokeratin
Structure Level Pattern Image Note
TS20: cap mesenchyme Weak Single cells 1N , 1P Expression is greatly reduced in the mutant, being limited to cells that escaped Cre recombination.
TS20: nephron Weak Single cells 1P , 1N Expression is greatly reduced in differentiating nephrons in the mutant, being limited to cells that escaped Cre recombination.
TS20: ureteric tip Present 1N , 1P
Specimen 1Q/S: embryonic day 13.5; Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Pax2; green - Cre; white - cytokeratin
Structure Level Pattern Image Note
TS21: cap mesenchyme Present 1Q , 1S
TS21: nephron Present 1Q , 1S Expression is present in differentiating nephrons.
TS21: collecting duct Present 1S , 1Q
TS21: ureteric tip Present 1S , 1Q
Specimen 1R/T: embryonic day 13.5; Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Pax2; green - Cre; white - cytokeratin
Structure Level Pattern Image Note
TS21: cap mesenchyme Absent 1R , 1T Expression is seen in a few, rare cells.
TS21: nephron Weak Single cells 1T , 1R Expression is greatly reduced in differentiating nephrons in the mutant, being limited to cells that escaped Cre recombination.
TS21: collecting duct Present 1R , 1T
TS21: ureteric tip Present 1R , 1T
Specimen not shown: embryonic day 19.5; Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: nephron Weak Expression is present in a new nephrons that escaped Cre recombination.
Specimen 2G: embryonic day 18.5; Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Pax2; red - Pdgfrb (assay MGI:6711802); white - cytokeratin
Structure Level Pattern Image Note
TS26: cap mesenchyme Present 2G
TS26: nephron epithelium Present 2G Expression is present in the epithelium of differentiating nephrons.
TS26: collecting duct Present 2G
TS26: ureteric tip Present 2G
TS26: renal cortical interstitium Absent 2G
Specimen 2H: embryonic day 18.5; Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Pax2; red - Pdgfrb (assay MGI:6711802); white - cytokeratin
Structure Level Pattern Image Note
TS26: cap mesenchyme Absent 2H
TS26: nephron epithelium Absent 2H
TS26: collecting duct Present 2H
TS26: renal cortical interstitium Absent 2H
Specimen 4A: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Pax2; red - Cre reporter; white - cytokeratin
Structure Level Pattern Image Note
TS26: renal cortex Present 4A
Specimen 4B: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Pax2; red - Cre reporter; white - cytokeratin
Structure Level Pattern Image Note
TS26: renal cortex Absent 4B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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