Gene Expression Data
Assay Details
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Assay
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Reference:
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J:286303
Buckley DM, et al., Gbx1 and Gbx2 Are Essential for Normal Patterning and Development of Interneurons and Motor Neurons in the Embryonic Spinal Cord. J Dev Biol. 2020 Apr 1;8(2)
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6705108
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Gene symbol:
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Pax2
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Gene name:
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paired box 2
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Antibody:
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anti-Pax2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A:
embryonic day 13.5
(more  )
Note: Lumbar spinal cord.
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
3A
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Pax2 is a cell-type specific marker of dorsal inhibitory interneurons. At E13.5, immunopositive cells span the medio-lateral axis of the dorsal spinal cord.
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Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
3B
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At E13.5, the superficial layers of the dorsal horn were less-densely populated with Pax2-positive cells in the Gbx1 mutant.
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
3C
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At E13.5, the superficial layers of the dorsal horn were less-densely populated with Pax2-positive cells in the Gbx2 mutant.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
3D
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Like the individual Gbx-mutants, the double mutant displayed a reduction in the number of Pax2-positive cells.
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Specimen
3E:
embryonic day 14.5
(more )
Note: Lumbar spinal cord.
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
3F
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Pax2 expression within the most peripheral regions of the superficial laminae remained significantly reduced in the Gbx1 mutant compared to the age-matched control.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
3G
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Pax2 expression within the most peripheral regions of the superficial laminae remained significantly reduced in the Gbx2 mutant compared to the age-matched control.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
3H
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Like the individual Gbx-mutants, the double mutant displayed a reduction in the number of Pax2-positive cells.
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Analysis Tools