Gene Expression Data
Assay Details
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Assay
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Reference:
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J:286303
Buckley DM, et al., Gbx1 and Gbx2 Are Essential for Normal Patterning and Development of Interneurons and Motor Neurons in the Embryonic Spinal Cord. J Dev Biol. 2020 Apr 1;8(2)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6705107
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Gene symbol:
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Gbx1
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Gene name:
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gastrulation brain homeobox 1
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Probe:
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Gbx1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1I:
embryonic day 13.5
(more  )
Note: Lumbar spinal cord.
Specimen
1I:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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Specimen
1J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
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1J
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Gbx1 expression pattern in the mutant recapitulates that of the control embryo at E13.5.
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| TS21: spinal cord dorsal horn |
Present |
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1J
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Gbx1 is expressed more broadly and intensely thorughout the dorsal horn in Gbx2-/- mutants.
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Specimen
1K:
embryonic day 14.5
(more )
Note: Lumbar spinal cord.
Specimen
1K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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Specimen
1L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lumbar spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
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1L
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A significant increase in Gbx1 expression persisted in the spinal cord of Gbx2-/- mutants through E14.5 compared to control embryos.
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Analysis Tools