Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:286303
Buckley DM, et al., Gbx1 and Gbx2 Are Essential for Normal Patterning and Development of Interneurons and Motor Neurons in the Embryonic Spinal Cord. J Dev Biol. 2020 Apr 1;8(2)
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6705106
|
|
Gene symbol:
|
Gbx2
|
|
Gene name:
|
gastrulation brain homeobox 2
|
|
Probe:
|
Gbx2 probe5
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
1A:
embryonic day 13.5
(more  )
Note: Lumbar spinal cord.
Specimen
1A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
Specimen
1B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord mantle layer |
Present |
Regionally restricted |
1B
|
The expression pattern remained consistent in the Gbx1-/- at E13.5. However, expression was higher throughout laminae III-IV in the mutant.
|
Specimen
1C:
embryonic day 14.5
(more )
Note: Lumbar spinal cord.
Specimen
1C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
Specimen
1D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
1D
|
Persistent upregulation of Gbx2 expression in Gbx1-/- spinal cord was seen through E14.5.
|
Specimen
1E:
embryonic day 15.5
(more )
Note: Lumbar spinal cord.
Specimen
1E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
Specimen
1F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
Specimen
1G:
embryonic day 16.5
(more )
Note: Lumbar spinal cord.
Specimen
1G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
Specimen
1H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Gbx1tm1.1Stwt/Gbx1tm1.1Stwt
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Lumbar spinal cord.
|
|
Analysis Tools