Pklrem1(cre/ERT2)Hzhu
Endonuclease-mediated Allele Detail
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| Symbol: |
Pklrem1(cre/ERT2)Hzhu |
| Name: |
pyruvate kinase liver and red blood cell; endonuclease-mediated mutation 1, Hao Zhu |
| MGI ID: |
MGI:6704919 |
| Synonyms: |
Pklr-CreER |
| Gene: |
Pklr Location: Chr3:89043449-89054091 bp, + strand Genetic Position: Chr3, 39.01 cM
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| Alliance: |
Pklrem1(cre/ERT2)Hzhu page
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| Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Pklrem1(cre/ERT2)Hzhu expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Pklr (MGI:97604) |
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Mutation details: The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the mouse Pklr gene. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission.
(J:304477)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Pklr
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Pklr Mutation: |
25 strains or lines available
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| Original: |
J:304477 Wei Y, et al., Liver homeostasis is maintained by midlobular zone 2 hepatocytes. Science. 2021 Feb 26;371(6532) |
| All: |
2 reference(s) |
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Analysis Tools
