Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Apoc4em1(cre/ERT2)Hzhu
Name:	apolipoprotein C-IV; endonuclease-mediated mutation 1, Hao Zhu
MGI ID:	MGI:6704909
Synonyms:	Apoc4-CreER
Gene:	Apoc4  Location: Chr7:19412009-19415385 bp, - strand  Genetic Position: Chr7, 9.94 cM
Alliance:	Apoc4em1(cre/ERT2)Hzhu page

Strain of Origin:

C57BL/6J

Allele Type:    Endonuclease-mediated (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Apoc4em1(cre/ERT2)Hzhu expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Apoc4 (MGI:87878)   Mutation details: The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the pyruvate kinase liver and red blood cell locus (Apoc4) on chromosome 7. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission. (J:304477)

Activity:	Tissue activity of this recombinase allele
Driver:	Apoc4 (mouse) Summary of all recombinase alleles driven by Apoc4. Alliance mouse Apoc4 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Apoc4 Mutation:	11 strains or lines available

Original:	J:304477 Wei Y, et al., Liver homeostasis is maintained by midlobular zone 2 hepatocytes. Science. 2021 Feb 26;371(6532)
All:	2 reference(s)