Gdf5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:302550 Towler OW, et al., Dysregulated BMP signaling through ACVR1 impairs digit joint development in fibrodysplasia ossificans progressiva (FOP). Dev Biol. 2021 Feb;470:136-146
Assay type:	RNA in situ
MGI Accession ID:	MGI:6696137
Gene symbol:	Gdf5
Gene name:	growth differentiation factor 5

Probe:	Gdf5 cDNA11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5A ©

Specimen 5A: embryonic day 14.5; Acvr1^tm2.1Vlcg/Acvr1⁺ (more

)

Structure	Level	Image
TS22: hindlimb digit 1 interphalangeal joint	Present	5A
TS22: hindlimb digit 2 interphalangeal joint	Present	5A
TS22: hindlimb digit 3 interphalangeal joint	Present	5A
TS22: hindlimb digit 4 interphalangeal joint	Present	5A
TS22: hindlimb digit 5 interphalangeal joint	Present	5A

5B ©

Specimen 5B: embryonic day 14.5; Acvr1^tm2.1Vlcg/Acvr1⁺, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: hindlimb interphalangeal joint	Present	Regionally restricted	5B	Expression is in bands in digits 3 and 4, and are absent or diffuse in digits 1 and 2.
TS22: hindlimb digit 1	Strong	Regionally restricted	5B	Expression is at the digit periphery.
TS22: hindlimb digit 2	Strong	Regionally restricted	5B	Expression is at the digit periphery.
TS22: hindlimb digit 3	Strong	Regionally restricted	5B	Expression is at the digit periphery.
TS22: hindlimb digit 4	Strong	Regionally restricted	5B	Expression is at the digit periphery.
TS22: hindlimb digit 5	Strong	Regionally restricted	5B	Expression is at the digit periphery.
TS22: hindlimb digit 3 interphalangeal joint	Present		5B
TS22: hindlimb digit 4 interphalangeal joint	Present		5B
TS22: hindlimb digit 5 interphalangeal joint	Present	Regionally restricted	5B	Expression is as a single band, rather than two.
TS22: hindlimb digit 1 interphalangeal joint	Trace	Diffuse	5B	Expression is absent or diffuse.
TS22: hindlimb digit 2 interphalangeal joint	Trace	Diffuse	5B	Expression is absent or diffuse.

S6A ©

Specimen S6A: embryonic day 15.5; Acvr1^tm2.1Vlcg/Acvr1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 1 interphalangeal joint	Present		S6A

S6B ©

Specimen S6B: embryonic day 15.5; Acvr1^tm2.1Vlcg/Acvr1⁺, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 1	Present	Regionally restricted	S6B	Expression is at the digit periphery.
TS23: hindlimb digit 1 interphalangeal joint	Absent		S6B

S6C ©

Specimen S6C: embryonic day 15.5; Acvr1^tm2.1Vlcg/Acvr1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 3 interphalangeal joint	Present		S6C

S6D ©

Specimen S6D: embryonic day 15.5; Acvr1^tm2.1Vlcg/Acvr1⁺, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 3 interphalangeal joint	Present		S6D

S6E ©

Specimen S6E: embryonic day 15.5; Acvr1^tm2.1Vlcg/Acvr1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 5 interphalangeal joint	Present		S6E

S6F ©

Specimen S6F: embryonic day 15.5; Acvr1^tm2.1Vlcg/Acvr1⁺, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 5	Present	Regionally restricted	S6F	Expression is at the digit periphery.
TS23: hindlimb digit 5 interphalangeal joint	Present		S6F

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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