About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:302550 Towler OW, et al., Dysregulated BMP signaling through ACVR1 impairs digit joint development in fibrodysplasia ossificans progressiva (FOP). Dev Biol. 2021 Feb;470:136-146
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6696061
Gene symbol: Smad1
Gene name: SMAD family member 1
Antibody: anti-phospho-Smad1/5/9 (13820)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Assay notes: The antibody used recognizes the phosphorylated form of the protein. Both Smad1 and Smad5 are annotated.
Results
Specimen 4A: embryonic day 12.0 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS20: forelimb digit Present Regionally restricted 4A There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS20: hand pre-cartilage condensation Present 4A
Specimen 4B: embryonic day 12.0; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: forelimb digit Present 4B This mutant showed reduced restriction of staining compared to controls.
Specimen 4C: embryonic day 12.0 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS20: hindlimb digit Present Regionally restricted 4C There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS20: foot pre-cartilage condensation Present 4C
Specimen 4D: embryonic day 12.0; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hindlimb digit Present 4D This mutant showed reduced restriction of staining compared to controls.
Specimen 4E: embryonic day 12.5 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS20: forelimb digit Present Regionally restricted 4E There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS20: hand pre-cartilage condensation Present 4E
Specimen 4F: embryonic day 12.5; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: forelimb digit Present 4F This mutant showed reduced restriction of staining compared to controls.
Specimen 4G: embryonic day 12.5 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS20: hindlimb digit Present Regionally restricted 4G There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS20: foot pre-cartilage condensation Present 4G
Specimen 4H: embryonic day 12.5; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hindlimb digit Present 4H This mutant showed reduced restriction of staining compared to controls.
Specimen 4I: embryonic day 13.0 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 4I There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS21: hand pre-cartilage condensation Present 4I
TS21: hand joint Absent 4I
Specimen 4J: embryonic day 13.0; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb digit Present 4J This mutant showed reduced restriction of staining compared to controls.
Specimen 4K: embryonic day 13.0 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS21: hindlimb digit Present Regionally restricted 4K There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS21: hindlimb digit pre-cartilage condensation Present 4K
TS21: foot joint Absent 4K
Specimen 4L: embryonic day 13.0; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: hindlimb digit Present 4L This mutant showed reduced restriction of staining compared to controls.
Specimen 4M: embryonic day 13.5 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 4M There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS21: hand pre-cartilage condensation Present 4M
TS21: hand joint Absent 4M
Specimen 4N: embryonic day 13.5; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb digit Present 4N This mutant showed reduced restriction of staining compared to controls.
Specimen 4O: embryonic day 13.5 (more )
Note: Controls were pooled wild type and single heterozygotes; the authors state that no differences were observed among control genotypes.
Structure Level Pattern Image Note
TS21: hindlimb digit Present Regionally restricted 4O There is an proximal-to-distal alternating pattern of positive and negative staining corresponding to regions of positive bone and negative joint.
TS21: hindlimb digit pre-cartilage condensation Present 4O
TS21: foot joint Absent 4O
Specimen 4P: embryonic day 13.5; Acvr1tm2.1Vlcg/Acvr1+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: hindlimb digit Present Regionally restricted 4P This mutant showed some restriction of staining.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory