Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:293790 Lewis EMA, et al., Geminin is required for Hox gene regulation to pattern the developing limb. Dev Biol. 2020 Aug 1;464(1):11-23
Assay type:	RNA in situ
MGI Accession ID:	MGI:6694286
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe195
Probe preparation:	Antisense RNA

Results

4_Shh-1 ©

4_Shh-2 ©

Specimen 4 cont: embryonic day 11.5; Gmnn^{tm1c(KOMP)Wtsi}/Gmnn⁺, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		4_Shh-1 , 4_Shh-2

4_Shh-3 ©

4_Shh-4 ©

Specimen 4 mut: embryonic day 11.5; Gmnn^{tm1c(KOMP)Wtsi}/Gmnn^{tm1c(KOMP)Wtsi}, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		4_Shh-4 , 4_Shh-3	Similar as in the control.

6A-1 ©

6A-2 ©

6A-3 ©

Specimen 6A cont: embryonic day 11.5; Gmnn^{tm1c(KOMP)Wtsi}/Gmnn⁺, Twist2^tm1(cre)Dor/Twist2⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6A-1 , 6A-3 , 6A-2

6A-4 ©

6A-5 ©

6A-6 ©

Specimen 6A mut: embryonic day 11.5; Gmnn^{tm1c(KOMP)Wtsi}/Gmnn^{tm1c(KOMP)Wtsi}, Twist2^tm1(cre)Dor/Twist2⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6A-5 , 6A-6 , 6A-4	Mutant had ectopic domain of expression in the anterior hindlimb bud with partial penetrance (3/8 embryos assayed).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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